Detailed information of TA system TA090507

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3763075..3763754
• Replicon: chromosome
• Accession: NZ_CP025520
• Organism: Escherichia coli strain DH5alpha

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: CDN75_RS19520
• Protein ID: WP_000854672.1
• Coordinates: 3763075..3763416 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: CDN75_RS19525
• Protein ID: WP_000070395.1
• Coordinates: 3763437..3763754 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDN75_RS19490	3758173..3759381	-	1209	WP_001352368.1	IS4-like element ISVsa5 family transposase	-
CDN75_RS19500	3759488..3759847	-	360	Protein_3545	porin	-
CDN75_RS19505	3760135..3761238	+	1104	WP_001285288.1	glutamate 5-kinase	-
CDN75_RS19510	3761250..3762503	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
CDN75_RS19520	3763075..3763416	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
CDN75_RS19525	3763437..3763754	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
CDN75_RS19530	3763773..3763994	-	222	WP_000691994.1	DUF987 domain-containing protein	-
CDN75_RS19535	3764003..3764479	-	477	WP_000811693.1	RadC family protein	-
CDN75_RS19540	3764495..3764953	-	459	WP_000211838.1	antirestriction protein	-
CDN75_RS19545	3765051..3765290	-	240	WP_000194654.1	DUF905 domain-containing protein	-
CDN75_RS19550	3765367..3765834	-	468	WP_001547765.1	hypothetical protein	-
CDN75_RS19555	3765857..3766300	-	444	WP_000824223.1	hypothetical protein	-
CDN75_RS19560	3766300..3766527	-	228	WP_001548158.1	hypothetical protein	-
CDN75_RS19565	3766523..3766714	-	192	Protein_3557	DeoR family transcriptional regulator	-
CDN75_RS19570	3766931..3767752	-	822	WP_000197389.1	DUF945 domain-containing protein	-
CDN75_RS19575	3767844..3768707	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T90507 WP_000854672.1 NZ_CP025520:c3763416-3763075 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T90507 NZ_CP025520:c3763416-3763075 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT90507 WP_000070395.1 NZ_CP025520:c3763754-3763437 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT90507 NZ_CP025520:c3763754-3763437 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684