Detailed information of TA system TA090500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 3388661..3389475
• Replicon: chromosome
• Accession: NZ_CP025520
• Organism: Escherichia coli strain DH5alpha

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: CDN75_RS17635
• Protein ID: WP_001054376.1
• Coordinates: 3389218..3389475 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: CDN75_RS17630
• Protein ID: WP_001309181.1
• Coordinates: 3388661..3389206 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDN75_RS17605	3384352..3385665	-	1314	WP_000460842.1	galactitol-specific PTS transporter subunit IIC	-
CDN75_RS17610	3385677..3385955	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
CDN75_RS17615	3385952..3387073	-	1122	WP_000010829.1	M42 family metallopeptidase	-
CDN75_RS24285	3387318..3387434	-	117	Protein_3202	VOC family protein	-
CDN75_RS17620	3387472..3387690	-	219	Protein_3203	hypothetical protein	-
CDN75_RS17625	3387859..3388605	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
CDN75_RS17630	3388661..3389206	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
CDN75_RS17635	3389218..3389475	-	258	WP_001054376.1	hypothetical protein	Toxin
CDN75_RS17645	3389852..3390097	-	246	Protein_3207	GNAT family N-acetyltransferase	-
CDN75_RS17655	3390213..3391453	+	1241	Protein_3208	helicase YjhR	-
CDN75_RS17660	3392036..3393016	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
CDN75_RS17665	3393081..3394187	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T90500 WP_001054376.1 NZ_CP025520:c3389475-3389218 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T90500 NZ_CP025520:c3389475-3389218 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT90500 WP_001309181.1 NZ_CP025520:c3389206-3388661 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT90500 NZ_CP025520:c3389206-3388661 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8