Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1892505..1893159 Replicon chromosome
Accession NZ_CP025520
Organism Escherichia coli strain DH5alpha

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag CDN75_RS09900 Protein ID WP_000244777.1
Coordinates 1892505..1892912 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CDN75_RS09905 Protein ID WP_000354046.1
Coordinates 1892893..1893159 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDN75_RS09880 1888462..1890195 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
CDN75_RS09885 1890201..1890911 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CDN75_RS09890 1890936..1891832 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CDN75_RS09895 1891944..1892465 + 522 WP_001055874.1 flavodoxin FldB -
CDN75_RS09900 1892505..1892912 - 408 WP_000244777.1 toxin CptA Toxin
CDN75_RS09905 1892893..1893159 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CDN75_RS09910 1893401..1894381 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
CDN75_RS09915 1894577..1895236 - 660 WP_000250274.1 hemolysin III family protein -
CDN75_RS09920 1895400..1895711 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
CDN75_RS09925 1895756..1897189 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
CDN75_RS09930 1897246..1897989 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T90492 WP_000244777.1 NZ_CP025520:c1892912-1892505 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T90492 NZ_CP025520:c1892912-1892505 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT90492 WP_000354046.1 NZ_CP025520:c1893159-1892893 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT90492 NZ_CP025520:c1893159-1892893 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References