Detailed information of TA system TA090457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 429559..430229
• Replicon: plasmid pRlvB
• Accession: NZ_CP025508
• Organism: Rhizobium leguminosarum bv. viciae strain UPM791

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: RLV_RS12670
• Protein ID: WP_018246604.1
• Coordinates: 429559..429963 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A444HGU5
• Locus tag: RLV_RS12675
• Protein ID: WP_018246605.1
• Coordinates: 429960..430229 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RLV_RS12635	424559..425080	+	522	WP_018246596.1	GNAT family N-acetyltransferase	-
RLV_RS12640	425123..425476	+	354	WP_018517210.1	type II toxin-antitoxin system RelE/ParE family toxin	-
RLV_RS12645	425491..425805	-	315	WP_011655201.1	helix-turn-helix domain-containing protein	-
RLV_RS12650	426321..427058	+	738	WP_026188781.1	hypothetical protein	-
RLV_RS38600	427065..427226	-	162	WP_018246599.1	hypothetical protein	-
RLV_RS12655	428044..428604	-	561	WP_018246601.1	hypothetical protein	-
RLV_RS12665	429164..429505	+	342	WP_018246603.1	hypothetical protein	-
RLV_RS12670	429559..429963	-	405	WP_018246604.1	type II toxin-antitoxin system VapC family toxin	Toxin
RLV_RS12675	429960..430229	-	270	WP_018246605.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
RLV_RS12680	430451..430669	-	219	WP_003547964.1	hypothetical protein	-
RLV_RS12685	430730..431575	-	846	WP_018517211.1	pirin family protein	-
RLV_RS12690	431671..433431	-	1761	WP_018246607.1	MOSC domain-containing protein	-
RLV_RS12695	433484..434017	-	534	WP_018246608.1	mismatch-specific DNA-glycosylase	-
RLV_RS12700	434160..434645	+	486	WP_026188782.1	DMT family transporter	-
RLV_RS12705	434642..435082	+	441	WP_018246610.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..597290	597290

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-117)

Antitoxin

• SpoVT-AbrB domain (18-53)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14188.56 Da
• Isoelectric Point: 5.1421

>T90457 WP_018246604.1 NZ_CP025508:c429963-429559 [Rhizobium leguminosarum bv. viciae]
VSRLYMLDTNIVSELARNPMGTITRRIAEVGPDAICVSIITASELRYGCAKKGSPKLLAQIEAILGSIPVLALDLPADAE
YGGIRAELEGAGKPTGPNDLFIAAHASALGAVLVTANMGEFMRIRALQVENWLI

Nucleotide

• Length: 405 bp

>T90457 NZ_CP025508:c429963-429559 [Rhizobium leguminosarum bv. viciae]
GTGAGCAGGCTTTATATGCTGGACACCAATATCGTCTCGGAGCTTGCTCGAAACCCGATGGGCACAATCACCCGACGCAT
CGCCGAGGTTGGGCCCGATGCAATTTGCGTCAGCATTATCACGGCATCGGAGTTGCGCTACGGGTGCGCCAAGAAGGGAT
CACCGAAACTTCTAGCACAGATCGAAGCCATCCTTGGCAGCATACCGGTGCTTGCGCTGGATCTGCCCGCCGATGCTGAA
TACGGTGGCATCCGGGCTGAACTGGAGGGTGCTGGCAAGCCTACCGGTCCAAATGACTTATTCATCGCTGCCCACGCCTC
TGCCCTCGGAGCAGTTCTGGTGACGGCGAACATGGGCGAGTTCATGCGTATCCGCGCTTTGCAGGTCGAAAACTGGTTGA
TTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10200.61 Da
• Isoelectric Point: 4.5610

>AT90457 WP_018246605.1 NZ_CP025508:c430229-429960 [Rhizobium leguminosarum bv. viciae]
VVNSRRQEPIPQREAKLFRNNKSQAVRIPADFELPGDRVMIHRDGDRLIIEPVHRKNLLEVLAGLEPLGPEDQFPDVDDT
LLPVEEIDL

Nucleotide

• Length: 270 bp

>AT90457 NZ_CP025508:c430229-429960 [Rhizobium leguminosarum bv. viciae]
GTGGTCAATTCCCGACGTCAAGAGCCTATTCCCCAGCGGGAGGCCAAGCTTTTCAGAAACAACAAGAGTCAGGCTGTACG
AATCCCGGCTGACTTTGAGCTACCCGGTGACAGAGTGATGATCCACCGCGACGGCGATCGGCTCATTATAGAGCCTGTGC
ACCGGAAGAACCTCCTGGAAGTTCTGGCTGGTCTGGAGCCGCTTGGTCCTGAAGATCAGTTTCCCGATGTCGACGATACC
CTGCTTCCTGTGGAGGAAATCGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A444HGU5