Detailed information of TA system TA090455

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 771874..772454
• Replicon: plasmid pRlvA
• Accession: NZ_CP025507
• Organism: Rhizobium leguminosarum bv. viciae strain UPM791

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A444I7Z5
• Locus tag: RLV_RS08175
• Protein ID: WP_018245324.1
• Coordinates: 771874..772257 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7Y3WNA0
• Locus tag: RLV_RS08180
• Protein ID: WP_018245323.1
• Coordinates: 772254..772454 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RLV_RS08145	767322..767542	-	221	Protein_721	Gfo/Idh/MocA family oxidoreductase	-
RLV_RS08155	767958..769052	+	1095	WP_018245327.1	hypothetical protein	-
RLV_RS08160	769349..770401	-	1053	WP_018245326.1	ribosome small subunit-dependent GTPase A	-
RLV_RS38540	770436..770612	-	177	WP_018071841.1	hypothetical protein	-
RLV_RS38165	770789..771118	+	330	WP_018517229.1	hypothetical protein	-
RLV_RS08170	771437..771664	-	228	WP_085990123.1	CopG family transcriptional regulator	-
RLV_RS08175	771874..772257	-	384	WP_018245324.1	type II toxin-antitoxin system VapC family toxin	Toxin
RLV_RS08180	772254..772454	-	201	WP_018245323.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
RLV_RS38170	772619..772858	-	240	WP_155256963.1	hypothetical protein	-
RLV_RS08190	772991..773308	+	318	Protein_730	glycerol-3-phosphate dehydrogenase	-
RLV_RS08195	773457..773585	-	129	Protein_731	transposase domain-containing protein	-
RLV_RS08200	773648..774280	+	633	WP_018245319.1	DUF433 domain-containing protein	-
RLV_RS08205	774277..774651	+	375	WP_018245318.1	DUF5615 family PIN-like protein	-
RLV_RS08210	774723..776303	-	1581	WP_018245317.1	ABC transporter substrate-binding protein	-
RLV_RS08215	776425..777210	+	786	WP_018245316.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..1291920	1291920

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14117.31 Da
• Isoelectric Point: 9.1808

>T90455 WP_018245324.1 NZ_CP025507:c772257-771874 [Rhizobium leguminosarum bv. viciae]
VILADTSIWIDHFRHVDAELRTIIEDDRLLCHPAVVGELALGSLRDRGRVIAFLAAQRQAFVATHDEVMTMIDRHGIFSM
GIGYTDAHLLASILLDRRAALWTRDKRLQAAAKKAGASLHKPANARN

Nucleotide

• Length: 384 bp

>T90455 NZ_CP025507:c772257-771874 [Rhizobium leguminosarum bv. viciae]
GTGATACTGGCAGACACTTCTATATGGATCGATCATTTCCGCCATGTTGATGCGGAACTTCGGACGATCATTGAGGACGA
TCGTCTACTCTGCCATCCGGCTGTAGTTGGCGAACTGGCGCTTGGCAGCCTTCGAGATCGCGGGCGCGTGATAGCGTTTC
TGGCAGCCCAGCGCCAAGCGTTCGTCGCAACGCATGACGAAGTGATGACAATGATTGATCGTCACGGCATTTTCAGTATG
GGCATCGGCTATACAGATGCCCACTTGCTCGCCTCGATACTGCTTGATCGGCGAGCGGCGTTATGGACCAGGGACAAGCG
TCTTCAGGCGGCGGCCAAAAAGGCAGGCGCTTCATTGCATAAGCCGGCCAATGCTCGCAACTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7124.23 Da
• Isoelectric Point: 11.3760

>AT90455 WP_018245323.1 NZ_CP025507:c772454-772254 [Rhizobium leguminosarum bv. viciae]
MRSTINLDDTLMERARSLTGTKETAAIVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Nucleotide

• Length: 201 bp

>AT90455 NZ_CP025507:c772454-772254 [Rhizobium leguminosarum bv. viciae]
ATGCGATCGACTATCAATCTAGACGACACTCTCATGGAAAGGGCCAGGTCCCTGACCGGCACAAAAGAAACCGCTGCTAT
AGTCCGCCAGGCGTTGGAGACGCTTGTTCGAGTAGAGTCCGGAAAACGCCTCATCGCGCTCGGCGGAACTATGCCCGATG
CAGAGGCAGCTCCACGTCGCCGGAGCGCAGCTGCCAAGTGA

Structures

Toxin

• AlphaFold DB: A0A444I7Z5

Antitoxin

• AlphaFold DB: A0A7Y3WNA0