Detailed information of TA system TA090454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 143439..144241
• Replicon: plasmid pRlvA
• Accession: NZ_CP025507
• Organism: Rhizobium leguminosarum bv. viciae strain UPM791

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: -
• Locus tag: RLV_RS05185
• Protein ID: WP_018516793.1
• Coordinates: 143439..143966 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: A0A7Y3SJF5
• Locus tag: RLV_RS05190
• Protein ID: WP_018245607.1
• Coordinates: 143963..144241 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RLV_RS05170	138737..139861	-	1125	WP_018516791.1	DNA repair exonuclease	-
RLV_RS05175	140151..141542	-	1392	WP_026188694.1	aldehyde dehydrogenase family protein	-
RLV_RS05180	141544..143190	-	1647	WP_018245609.1	acetolactate synthase large subunit	-
RLV_RS05185	143439..143966	-	528	WP_018516793.1	GNAT family N-acetyltransferase	Toxin
RLV_RS05190	143963..144241	-	279	WP_018245607.1	DUF1778 domain-containing protein	Antitoxin
RLV_RS05195	144396..145274	-	879	WP_026188693.1	hypothetical protein	-
RLV_RS05200	145598..147712	-	2115	WP_018245605.1	carboxy terminal-processing peptidase	-
RLV_RS05205	147860..148339	-	480	WP_020493431.1	MEKHLA domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..1291920	1291920

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (12-87)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 18814.79 Da
• Isoelectric Point: 9.5510

>T90454 WP_018516793.1 NZ_CP025507:c143966-143439 [Rhizobium leguminosarum bv. viciae]
VTLPVWHEEPIAKSHDRASFDCGDAAMNEFIRRFARQSHEQNAAKTFCAIDKAHPDRILGFYTVAPSAVTHEAVPPKMTR
GLARHEVAGFKLARLATDIKVAGKGLGGQLIAAAALRCLRLASEGGGILLIIDAKSERAAHWYASYGAEPLQGKPLTLVM
PLATFAADFKAKGLL

Nucleotide

• Length: 528 bp

>T90454 NZ_CP025507:c143966-143439 [Rhizobium leguminosarum bv. viciae]
GTGACCCTCCCCGTCTGGCATGAAGAGCCGATCGCTAAGTCGCATGATCGGGCCTCATTCGATTGCGGTGATGCGGCAAT
GAACGAGTTCATTCGGCGCTTCGCCCGGCAGAGCCATGAGCAGAATGCGGCGAAAACCTTCTGCGCTATCGACAAGGCCC
ACCCTGATCGCATCCTCGGATTCTACACAGTCGCGCCCTCTGCCGTTACGCATGAGGCCGTGCCTCCGAAGATGACGAGA
GGCCTCGCACGCCATGAAGTCGCAGGCTTCAAGCTCGCGCGCTTGGCAACCGACATAAAGGTGGCGGGAAAAGGTTTAGG
CGGTCAGCTCATCGCTGCTGCAGCGCTCCGTTGCCTGCGGCTCGCCAGCGAGGGCGGCGGGATCCTGCTAATCATCGATG
CCAAGAGCGAGCGCGCCGCCCATTGGTATGCCAGCTACGGCGCCGAACCGCTGCAAGGGAAACCGCTCACCCTCGTTATG
CCGCTTGCCACCTTCGCCGCCGACTTCAAGGCCAAGGGGCTCCTGTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10222.91 Da
• Isoelectric Point: 9.8602

>AT90454 WP_018245607.1 NZ_CP025507:c144241-143963 [Rhizobium leguminosarum bv. viciae]
MPRTADNKSERYQLRIPPKQKAMIARAAALSNKDMADFILDKIVPEAEAVIQAAEVETVSSRDFIRILDLLERPPKPNAR
LRAAISALPDAL

Nucleotide

• Length: 279 bp

>AT90454 NZ_CP025507:c144241-143963 [Rhizobium leguminosarum bv. viciae]
ATGCCGAGAACCGCGGACAATAAATCCGAACGCTACCAGCTGCGCATCCCGCCAAAGCAGAAGGCAATGATCGCGCGCGC
AGCTGCCCTTTCGAACAAGGATATGGCCGATTTCATTCTCGATAAGATAGTACCTGAAGCCGAAGCCGTCATCCAGGCTG
CTGAAGTTGAGACCGTTTCGAGCCGCGATTTCATCCGCATCCTCGACCTGCTTGAACGCCCGCCCAAACCAAACGCGAGA
CTACGCGCCGCGATCTCTGCTCTACCGGATGCCTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7Y3SJF5