Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rlegTA/AbiEii-AbiEi
Location 390873..392352 Replicon plasmid pRlvC
Accession NZ_CP025505
Organism Rhizobium leguminosarum bv. viciae strain UPM791

Toxin (Protein)


Gene name rlegT Uniprot ID A0A2L1CL59
Locus tag RLV_RS01785 Protein ID WP_018516728.1
Coordinates 390873..391742 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name rlegA Uniprot ID A0A2L1CLJ7
Locus tag RLV_RS01790 Protein ID WP_026242323.1
Coordinates 391735..392352 (-) Length 206 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RLV_RS01770 388285..388848 + 564 WP_018516731.1 conjugative transfer signal peptidase TraF -
RLV_RS01775 388838..390004 + 1167 WP_018516730.1 conjugal transfer protein TraB -
RLV_RS01780 390021..390638 + 618 WP_018516729.1 conjugal transfer protein TraH -
RLV_RS01785 390873..391742 - 870 WP_018516728.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
RLV_RS01790 391735..392352 - 618 WP_026242323.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein Antitoxin
RLV_RS01800 392680..392883 - 204 WP_018516726.1 DUF433 domain-containing protein -
RLV_RS01805 392935..393147 - 213 WP_018482035.1 helix-turn-helix transcriptional regulator -
RLV_RS01810 393353..393676 + 324 WP_018482034.1 transcriptional repressor TraM -
RLV_RS01815 393680..394382 - 703 Protein_349 autoinducer binding domain-containing protein -
RLV_RS01820 394694..395992 - 1299 WP_018516723.1 IncP-type conjugal transfer protein TrbI -
RLV_RS01825 396004..396444 - 441 WP_018516722.1 lipoprotein -
RLV_RS01830 396448..397257 - 810 WP_026242322.1 P-type conjugative transfer protein TrbG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..405209 405209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(38-263)

Antitoxin

Transcriptional regulator, AbiEi antitoxin

(15-56)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32344.07 Da        Isoelectric Point: 5.2218

>T90452 WP_018516728.1 NZ_CP025505:c391742-390873 [Rhizobium leguminosarum bv. viciae]
MAKEIRNVGASVRARLLQLAKASGQSFDLVLTRFALERLLFRLSQSPHAGRFVLKGAMLMMSWFDNPHRGTRDLDLLGFG
DPSPDPMLETFRGILAQEADDGVTFDADTLHVDRIREALDYGGLRLRVIASISGARINLTIDIGFGDALEPGVEVLDYPS
MLDFPMPRLRAYARETVIAEKFQAMVMLGRVNSRMKDFYDIWILSRSFDFGDDRLARAIAATFDRRETSIPIDLPDALTD
AFAKDQQKQRQWRAFVEGVAHNPGDLTDVIAEIATFLMPHAVAAARLDE

Download         Length: 870 bp

>T90452 NZ_CP025505:c391742-390873 [Rhizobium leguminosarum bv. viciae]
ATGGCTAAAGAAATCAGAAACGTCGGCGCCTCGGTGCGCGCCCGCCTGTTGCAACTCGCCAAGGCAAGCGGGCAAAGCTT
CGATCTGGTCCTGACGCGTTTTGCTCTTGAACGGTTATTGTTCCGGCTTAGCCAGTCGCCCCACGCCGGTCGCTTCGTTC
TAAAAGGGGCGATGTTGATGATGAGTTGGTTCGACAATCCGCACCGAGGCACGCGCGACCTCGATCTTTTGGGTTTCGGT
GATCCGAGTCCGGACCCGATGCTTGAGACATTTCGGGGAATTCTGGCGCAAGAGGCCGACGATGGCGTTACGTTCGACGC
TGATACGCTGCATGTGGATCGTATTCGCGAGGCATTGGATTATGGTGGGCTCAGGCTGCGGGTGATCGCTTCGATCAGCG
GAGCCCGGATTAACCTGACGATCGACATCGGTTTTGGCGATGCGCTTGAGCCTGGGGTGGAGGTTTTGGATTATCCTTCC
ATGCTCGATTTTCCGATGCCGCGGCTTCGGGCCTATGCGCGAGAAACGGTCATTGCCGAGAAGTTCCAGGCAATGGTGAT
GCTGGGGCGAGTAAACAGCCGCATGAAGGATTTCTACGACATCTGGATCCTCAGCAGGTCTTTCGACTTCGGCGATGATC
GGCTGGCGCGCGCAATAGCCGCTACCTTTGATCGCCGCGAGACATCGATCCCCATTGACCTCCCCGACGCCCTAACGGAC
GCCTTTGCCAAGGACCAGCAAAAGCAGCGGCAATGGCGAGCTTTCGTTGAAGGCGTTGCACACAATCCTGGCGATCTGAC
AGACGTCATCGCGGAGATTGCCACATTCCTGATGCCGCACGCGGTTGCGGCTGCGAGGCTGGACGAATGA

Antitoxin


Download         Length: 206 a.a.        Molecular weight: 22346.95 Da        Isoelectric Point: 10.2141

>AT90452 WP_026242323.1 NZ_CP025505:c392352-391735 [Rhizobium leguminosarum bv. viciae]
MPIPVTQRQIARTVLTERGIARLVELRNAGVTAATMSRMERDGEVLRLARGLYQLPDAQLDVNHSLAEVAKRAPKGVICL
VSALAFHGLTDQLPGQIWLAIGRKDWAPKLDGPAMRIVRFTDGLLNESVEPHVIEGVPVRVFGIAKTIADCFRYRNKIGL
SVAIEGLQEALRQRRATPGEIANQAERGGVGSVIRPYLEALTANG

Download         Length: 618 bp

>AT90452 NZ_CP025505:c392352-391735 [Rhizobium leguminosarum bv. viciae]
ATGCCCATTCCCGTCACGCAACGCCAAATTGCCCGCACCGTGCTAACTGAGCGCGGAATAGCACGTTTGGTGGAACTGCG
GAACGCAGGTGTGACGGCTGCCACCATGAGCCGAATGGAACGAGATGGTGAAGTGCTCCGGCTTGCCCGCGGTCTTTATC
AACTTCCTGATGCGCAGCTCGACGTCAACCACAGCCTGGCGGAGGTTGCCAAACGCGCACCCAAAGGCGTTATCTGCCTC
GTTTCGGCACTGGCGTTCCACGGCCTGACAGATCAGCTCCCAGGACAAATCTGGCTTGCCATAGGCCGTAAGGACTGGGC
TCCGAAACTGGACGGCCCCGCTATGCGCATCGTGCGTTTCACAGACGGCCTTCTCAACGAAAGCGTCGAACCCCATGTCA
TTGAAGGCGTTCCCGTGAGGGTCTTTGGAATTGCCAAGACAATTGCCGATTGCTTTCGCTATCGCAACAAGATCGGCCTT
TCGGTAGCGATAGAAGGGCTGCAGGAGGCGCTGCGGCAACGAAGGGCTACTCCTGGCGAAATCGCCAACCAGGCCGAACG
CGGTGGCGTCGGATCGGTGATCCGGCCGTATCTCGAGGCGCTGACCGCCAATGGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1CL59

Antitoxin

Source ID Structure
AlphaFold DB A0A2L1CLJ7

References