Detailed information of TA system TA090449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higB-toxA/Tad-HTH_37
• Location: 448856..449484
• Replicon: chromosome
• Accession: NZ_CP025499
• Organism: Lacticaseibacillus paracasei subsp. tolerans strain AO356

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A806L3G4
• Locus tag: CYL78_RS02710
• Protein ID: WP_012490993.1
• Coordinates: 449116..449484 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: CYL78_RS02705
• Protein ID: WP_003583264.1
• Coordinates: 448856..449116 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CYL78_RS02680	444747..445490	+	744	WP_012490990.1	SDR family oxidoreductase	-
CYL78_RS02685	445603..446229	+	627	WP_003577713.1	flavodoxin	-
CYL78_RS02690	446480..446983	+	504	WP_012490991.1	TetR/AcrR family transcriptional regulator	-
CYL78_RS02695	446988..448049	+	1062	WP_003563374.1	ABC transporter permease	-
CYL78_RS02700	448054..448743	+	690	WP_012490992.1	ABC transporter ATP-binding protein	-
CYL78_RS02705	448856..449116	-	261	WP_003583264.1	helix-turn-helix transcriptional regulator	Antitoxin
CYL78_RS02710	449116..449484	-	369	WP_012490993.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CYL78_RS02715	449775..451145	-	1371	WP_012490994.1	FAD-dependent oxidoreductase	-
CYL78_RS02720	451509..452327	-	819	WP_012490995.1	Cof-type HAD-IIB family hydrolase	-
CYL78_RS02725	452643..452912	-	270	WP_012490996.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
CYL78_RS02730	453153..454160	+	1008	WP_003563383.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (34-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (28-79)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 14970.02 Da
• Isoelectric Point: 8.3986

>T90449 WP_012490993.1 NZ_CP025499:c449484-449116 [Lacticaseibacillus paracasei subsp. tolerans]
MYKIDFYEDRDGYSEIEDFLDQLRHSHQKNNVSLLNKITKELYMLQNLGPRLREPHAKFLKGYAYPIMELRPMQERIFYA
AWQKDHFVLLHHYTKKTNKTDHREILRAQSNLEDWLSRKEEN

Nucleotide

• Length: 369 bp

>T90449 NZ_CP025499:c449484-449116 [Lacticaseibacillus paracasei subsp. tolerans]
ATGTATAAAATTGACTTCTATGAGGATCGCGATGGCTACTCTGAGATCGAAGACTTTCTTGATCAGTTACGCCATAGTCA
TCAAAAAAATAATGTGAGCCTTTTAAACAAAATCACAAAGGAACTCTACATGCTGCAAAATCTTGGTCCGCGCTTACGTG
AACCTCACGCGAAGTTTCTGAAGGGATATGCTTATCCGATCATGGAATTGCGTCCCATGCAAGAAAGAATCTTTTATGCT
GCATGGCAAAAGGATCATTTCGTTTTGCTTCATCACTACACCAAGAAAACAAACAAAACTGATCACAGAGAAATTTTACG
GGCACAGTCTAACCTCGAAGATTGGCTCTCTAGAAAGGAAGAAAATTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9828.45 Da
• Isoelectric Point: 10.2439

>AT90449 WP_003583264.1 NZ_CP025499:c449116-448856 [Lacticaseibacillus paracasei subsp. tolerans]
MTTWEDFSQKLKKDNPEKLAIIKTLADLESERIKQGISQKDFAEKIGMKQPQLAKIERLDSLPTLATLNRYARGLGKEIR
LSIVTQ

Nucleotide

• Length: 261 bp

>AT90449 NZ_CP025499:c449116-448856 [Lacticaseibacillus paracasei subsp. tolerans]
ATGACGACATGGGAAGACTTCAGTCAGAAACTGAAGAAGGACAATCCAGAAAAGTTGGCAATCATCAAAACCTTGGCCGA
TTTAGAAAGTGAACGAATCAAGCAAGGAATCTCTCAAAAGGACTTTGCAGAAAAAATTGGTATGAAACAGCCGCAACTAG
CAAAGATTGAGCGCTTAGACTCTCTTCCGACTCTGGCAACACTCAATCGATACGCGAGAGGGCTTGGAAAAGAAATTCGT
TTATCAATTGTTACTCAGTGA

Structures

Toxin

• AlphaFold DB: A0A806L3G4