Detailed information of TA system TA090442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2056691..2057220
• Replicon: chromosome
• Accession: NZ_CP025495
• Organism: Staphylococcus aureus strain 3020.C01

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: A7U46_RS10600
• Protein ID: WP_000621175.1
• Coordinates: 2056858..2057220 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: A7U46_RS10595
• Protein ID: WP_000948331.1
• Coordinates: 2056691..2056861 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7U46_RS10565	2051728..2052288	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
A7U46_RS10570	2052496..2052975	+	480	WP_001287088.1	hypothetical protein	-
A7U46_RS10575	2052968..2054551	+	1584	WP_001294626.1	PH domain-containing protein	-
A7U46_RS10580	2054538..2055029	+	492	WP_001205910.1	PH domain-containing protein	-
A7U46_RS10585	2055033..2055392	+	360	WP_000581200.1	holo-ACP synthase	-
A7U46_RS10590	2055458..2056606	+	1149	WP_001281145.1	alanine racemase	-
A7U46_RS10595	2056691..2056861	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
A7U46_RS10600	2056858..2057220	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
A7U46_RS10610	2057570..2058571	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
A7U46_RS10615	2058690..2059016	+	327	WP_001052491.1	anti-sigma factor antagonist	-
A7U46_RS10620	2059018..2059497	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
A7U46_RS10625	2059472..2060242	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T90442 WP_000621175.1 NZ_CP025495:2056858-2057220 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T90442 NZ_CP025495:2056858-2057220 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT90442 WP_000948331.1 NZ_CP025495:2056691-2056861 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT90442 NZ_CP025495:2056691-2056861 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1