Detailed information of TA system TA090431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 27126..27660
• Replicon: plasmid pLA01-117_150k
• Accession: NZ_CP025492
• Organism: Legionella sainthelensi strain LA01-117

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A7G4RL39
• Locus tag: CAB17_RS18835
• Protein ID: WP_014845122.1
• Coordinates: 27379..27660 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A7G4RL40
• Locus tag: CAB17_RS18830
• Protein ID: WP_014845121.1
• Coordinates: 27126..27386 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAB17_RS20180	22458..22613	-	156	WP_154080667.1	hypothetical protein	-
CAB17_RS18795	22685..23176	+	492	WP_051009158.1	IS91 family transposase	-
CAB17_RS18800	23198..23731	+	534	WP_061637559.1	IS91 family transposase	-
CAB17_RS18805	24097..24489	+	393	Protein_24	methyltransferase domain-containing protein	-
CAB17_RS18810	24927..25247	-	321	WP_014845117.1	type II toxin-antitoxin system PemK/MazF family toxin	-
CAB17_RS18815	25234..25467	-	234	WP_014845118.1	hypothetical protein	-
CAB17_RS18820	25576..25923	-	348	WP_080031944.1	WGR domain-containing protein	-
CAB17_RS18825	26029..26913	-	885	WP_014845120.1	recombinase family protein	-
CAB17_RS18830	27126..27386	+	261	WP_014845121.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
CAB17_RS18835	27379..27660	+	282	WP_014845122.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
CAB17_RS18840	27840..30338	-	2499	WP_014845123.1	DEAD/DEAH box helicase family protein	-
CAB17_RS18845	30387..30677	-	291	WP_014845124.1	helix-turn-helix domain-containing protein	-
CAB17_RS18850	30655..31008	-	354	WP_014845125.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CAB17_RS18855	31047..31865	-	819	WP_014845126.1	hypothetical protein	-
CAB17_RS18860	31918..32241	-	324	WP_014845127.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-29	-	1..150432	150432

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-92)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-74)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11028.69 Da
• Isoelectric Point: 8.9816

>T90431 WP_014845122.1 NZ_CP025492:27379-27660 [Legionella sainthelensi]
MLELELATQFKRDLKKITKQGKRRTLLDSIVEQLQKEEPLDPKHKDHSLSVNWDGYRECHITPDWLLIYKTIKDDRIQLL
RLSRTGSHSDLFG

Nucleotide

• Length: 282 bp

>T90431 NZ_CP025492:27379-27660 [Legionella sainthelensi]
ATGCTTGAATTAGAGTTGGCGACACAGTTTAAGCGTGATTTGAAAAAGATTACGAAGCAAGGGAAAAGAAGGACGCTCTT
AGACTCTATTGTAGAGCAATTGCAAAAGGAAGAACCCCTGGATCCAAAGCATAAGGATCACAGTCTTTCCGTAAATTGGG
ATGGTTATCGTGAATGTCACATTACCCCCGATTGGTTATTAATTTATAAGACCATTAAAGATGATCGCATACAATTGCTG
CGATTATCACGAACAGGATCACATAGTGATTTGTTTGGGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9594.08 Da
• Isoelectric Point: 6.9803

>AT90431 WP_014845121.1 NZ_CP025492:27126-27386 [Legionella sainthelensi]
MNKAATINARIEPALKMQAEAILHKVGLSTAEAIRLFYSQVCLQNGLPFEVKIPNKETREAMAELESGKGERFKTMKDVW
DSVDNA

Nucleotide

• Length: 261 bp

>AT90431 NZ_CP025492:27126-27386 [Legionella sainthelensi]
ATGAATAAGGCGGCAACAATTAATGCTCGTATTGAGCCTGCTCTTAAAATGCAGGCAGAAGCGATTTTGCATAAGGTAGG
CTTATCTACAGCCGAGGCGATTCGACTGTTCTATAGCCAGGTCTGTCTCCAAAATGGCCTGCCTTTTGAGGTAAAAATAC
CGAACAAAGAGACAAGAGAGGCCATGGCAGAGCTGGAATCAGGAAAAGGCGAGCGATTTAAAACAATGAAGGATGTATGG
GATTCTGTAGATAATGCTTGA

Structures

Toxin

• AlphaFold DB: A0A7G4RL39

Antitoxin

• AlphaFold DB: A0A7G4RL40