Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 24927..25467 Replicon plasmid pLA01-117_150k
Accession NZ_CP025492
Organism Legionella sainthelensi strain LA01-117

Toxin (Protein)


Gene name mazF Uniprot ID A0A7G4RL43
Locus tag CAB17_RS18810 Protein ID WP_014845117.1
Coordinates 24927..25247 (-) Length 107 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2H5FRI5
Locus tag CAB17_RS18815 Protein ID WP_014845118.1
Coordinates 25234..25467 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CAB17_RS18780 19939..20241 - 303 WP_014845112.1 transposase -
CAB17_RS18785 20867..21358 - 492 WP_014845113.1 Hsp20/alpha crystallin family protein -
CAB17_RS18790 21506..22093 - 588 WP_014845114.1 Hsp20/alpha crystallin family protein -
CAB17_RS20180 22458..22613 - 156 WP_154080667.1 hypothetical protein -
CAB17_RS18795 22685..23176 + 492 WP_051009158.1 IS91 family transposase -
CAB17_RS18800 23198..23731 + 534 WP_061637559.1 IS91 family transposase -
CAB17_RS18805 24097..24489 + 393 Protein_24 methyltransferase domain-containing protein -
CAB17_RS18810 24927..25247 - 321 WP_014845117.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CAB17_RS18815 25234..25467 - 234 WP_014845118.1 hypothetical protein Antitoxin
CAB17_RS18820 25576..25923 - 348 WP_080031944.1 WGR domain-containing protein -
CAB17_RS18825 26029..26913 - 885 WP_014845120.1 recombinase family protein -
CAB17_RS18830 27126..27386 + 261 WP_014845121.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
CAB17_RS18835 27379..27660 + 282 WP_014845122.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family -
CAB17_RS18840 27840..30338 - 2499 WP_014845123.1 DEAD/DEAH box helicase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaOXA-29 - 1..150432 150432


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 107 a.a.        Molecular weight: 11444.32 Da        Isoelectric Point: 9.4321

>T90430 WP_014845117.1 NZ_CP025492:c25247-24927 [Legionella sainthelensi]
MNRGDVYWVNLDPTTGSEINKLRPCVLVGATPINQARRTVVVVPLSSSATARPPITITVSCLGKQVTAVCDQIRTIDKSR
LKKVAGNLSDKDLNALDDGLRQVLCL

Download         Length: 321 bp

>T90430 NZ_CP025492:c25247-24927 [Legionella sainthelensi]
ATGAATCGTGGTGATGTATATTGGGTAAACCTCGACCCTACTACAGGCTCTGAAATCAACAAACTCAGACCTTGCGTTTT
AGTTGGAGCAACACCGATTAATCAAGCAAGACGCACTGTTGTCGTCGTTCCTCTTTCAAGCTCTGCTACAGCCAGACCAC
CAATTACCATCACTGTTTCTTGTCTTGGCAAACAAGTTACCGCAGTTTGTGATCAAATAAGAACGATTGATAAAAGTCGT
TTAAAAAAAGTGGCAGGAAACCTTTCAGATAAAGATTTAAATGCCTTGGATGATGGGTTACGACAAGTTTTATGCCTTTG
A

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8932.14 Da        Isoelectric Point: 4.8047

>AT90430 WP_014845118.1 NZ_CP025492:c25467-25234 [Legionella sainthelensi]
MNKVLISIPDQIASRMRAAIPQRQRSKVIAHLIEKEIERREKALYECALAVENDHGLQNEMNDWDITVQDGLTDESW

Download         Length: 234 bp

>AT90430 NZ_CP025492:c25467-25234 [Legionella sainthelensi]
ATGAATAAAGTTTTAATATCAATACCCGATCAAATCGCTTCTAGAATGAGAGCAGCAATACCTCAGCGACAACGCAGCAA
GGTGATTGCTCATCTTATTGAAAAAGAAATTGAACGACGTGAAAAAGCGCTCTATGAATGCGCTTTAGCTGTTGAAAATG
ATCATGGTTTACAAAATGAAATGAACGATTGGGATATAACGGTTCAAGATGGATTAACTGATGAATCGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G4RL43

Antitoxin

Source ID Structure
AlphaFold DB A0A2H5FRI5

References