Detailed information of TA system TA090416

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 128635..129130
• Replicon: chromosome
• Accession: NZ_CP025473
• Organism: Enterococcus mundtii strain Pe161

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CYK55_RS00995
• Protein ID: WP_080693167.1
• Coordinates: 128906..129130 (+)
• Length: 75 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A022N6L0
• Locus tag: CYK55_RS00990
• Protein ID: WP_034689371.1
• Coordinates: 128635..128868 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CYK55_RS00960	123918..124672	+	755	Protein_123	ATP-binding cassette domain-containing protein	-
CYK55_RS00965	124694..125647	+	954	WP_034689376.1	siderophore ABC transporter substrate-binding protein	-
CYK55_RS00970	125906..126202	-	297	WP_034689375.1	hypothetical protein	-
CYK55_RS00975	126422..127000	+	579	WP_034689374.1	GNAT family N-acetyltransferase	-
CYK55_RS00980	127048..127407	+	360	WP_010734414.1	iron-sulfur cluster biosynthesis family protein	-
CYK55_RS00985	127426..128322	+	897	WP_034689372.1	metallophosphoesterase	-
CYK55_RS00990	128635..128868	+	234	WP_034689371.1	toxin-antitoxin system antitoxin component	Antitoxin
CYK55_RS00995	128906..129130	+	225	WP_080693167.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CYK55_RS01000	129540..130274	-	735	WP_019723412.1	aquaporin family protein	-
CYK55_RS01005	130708..132237	+	1530	WP_034689369.1	helix-turn-helix domain-containing protein	-
CYK55_RS01010	132227..132448	+	222	WP_010734410.1	DUF2829 domain-containing protein	-
CYK55_RS01015	132445..133206	+	762	WP_034689367.1	3-oxoacyl-ACP reductase	-
CYK55_RS01020	133235..133873	+	639	WP_034689365.1	DUF998 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (9-73)

Antitoxin

• Protein of unknown function DUF6290 (1-63)

Sequences

Toxin

Protein

• Length: 75 a.a.
• Molecular weight: 8743.21 Da
• Isoelectric Point: 10.5505

>T90416 WP_080693167.1 NZ_CP025473:128906-129130 [Enterococcus mundtii]
IRKLDKFEATTILRWLHKQNEGTKNPGYSGKPLVGNYTVKWRYRIGNYRVIAKIDDEKLIVLTLEVGHRKNIYL

Nucleotide

• Length: 225 bp

>T90416 NZ_CP025473:128906-129130 [Enterococcus mundtii]
ATAAGAAAACTAGACAAATTCGAAGCAACAACTATTTTAAGATGGTTACATAAACAGAACGAAGGCACAAAAAATCCTGG
ATATTCAGGTAAGCCACTTGTGGGGAACTACACAGTTAAATGGCGCTACAGAATAGGAAATTATCGCGTCATTGCAAAGA
TTGATGATGAAAAGTTGATTGTTTTAACATTAGAAGTAGGACATAGAAAAAATATATATCTATAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8791.12 Da
• Isoelectric Point: 4.4189

>AT90416 WP_034689371.1 NZ_CP025473:128635-128868 [Enterococcus mundtii]
MVTITVRVTEDEKKFLDEMAKFEGKSLSALLKTTILNVLEDAYDAKIGDLAYEEYLKDKKSNDLSVLLEEYEIGVKK

Nucleotide

• Length: 234 bp

>AT90416 NZ_CP025473:128635-128868 [Enterococcus mundtii]
ATGGTAACAATTACAGTTCGTGTGACAGAAGATGAGAAAAAATTTTTGGATGAAATGGCTAAATTTGAAGGAAAAAGTCT
CTCTGCATTACTTAAAACAACAATATTAAATGTTTTAGAAGATGCGTATGATGCAAAGATAGGAGATTTAGCCTATGAAG
AGTATTTGAAAGATAAAAAATCAAATGATTTATCTGTTCTTTTAGAGGAGTATGAGATAGGTGTAAAAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A022N6L0