Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 42323..42966 Replicon plasmid p44-2
Accession NZ_CP025463
Organism Klebsiella pneumoniae strain F44

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag CYE02_RS29745 Protein ID WP_001044770.1
Coordinates 42550..42966 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag CYE02_RS29740 Protein ID WP_001261282.1
Coordinates 42323..42553 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CYE02_RS29705 37546..38325 - 780 WP_013214009.1 site-specific integrase -
CYE02_RS29710 38509..39513 - 1005 WP_011977814.1 hypothetical protein -
CYE02_RS29715 39543..39746 - 204 WP_011977813.1 hypothetical protein -
CYE02_RS29720 39792..40313 - 522 WP_013214008.1 hypothetical protein -
CYE02_RS29725 40371..40763 - 393 WP_011977811.1 hypothetical protein -
CYE02_RS29730 40800..41744 - 945 WP_011977810.1 hypothetical protein -
CYE02_RS31675 41905..42366 - 462 WP_014343465.1 hypothetical protein -
CYE02_RS29740 42323..42553 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CYE02_RS29745 42550..42966 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
CYE02_RS29750 43040..44602 + 1563 WP_004206609.1 AAA family ATPase -
CYE02_RS29755 44587..45609 + 1023 WP_000361404.1 helicase UvrD -
CYE02_RS29760 45865..46562 + 698 Protein_62 IS1 family transposase -
CYE02_RS29765 46591..46863 + 273 Protein_63 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / blaCTX-M-65 / catA2 - 1..161580 161580


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T90386 WP_001044770.1 NZ_CP025463:42550-42966 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T90386 NZ_CP025463:42550-42966 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT90386 WP_001261282.1 NZ_CP025463:42323-42553 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT90386 NZ_CP025463:42323-42553 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References