Detailed information of TA system TA090385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 214716..215386
• Replicon: plasmid p44-1
• Accession: NZ_CP025462
• Organism: Klebsiella pneumoniae strain F44

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: CYE02_RS29085
• Protein ID: WP_004213072.1
• Coordinates: 214943..215386 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: CYE02_RS29080
• Protein ID: WP_004213073.1
• Coordinates: 214716..214946 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CYE02_RS29055	210939..211838	-	900	WP_004225022.1	nucleotide-binding protein	-
CYE02_RS29060	211828..212118	-	291	WP_004213078.1	hypothetical protein	-
CYE02_RS29065	212470..212676	+	207	WP_004213077.1	hypothetical protein	-
CYE02_RS29070	212666..212959	-	294	WP_004213076.1	hypothetical protein	-
CYE02_RS29075	212975..214108	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
CYE02_RS29080	214716..214946	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CYE02_RS29085	214943..215386	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
CYE02_RS29090	215400..215816	-	417	Protein_223	restriction endonuclease	-
CYE02_RS29095	215820..216113	-	294	Protein_224	transposase	-
CYE02_RS31660	216530..217164	+	635	Protein_225	response regulator	-
CYE02_RS29115	217679..218085	-	407	Protein_226	GAF domain-containing protein	-
CYE02_RS29125	218176..219096	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
CYE02_RS29130	219145..219636	-	492	WP_011251327.1	DM13 domain-containing protein	-
CYE02_RS29135	219699..219974	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / blaTEM-1B / aac(3)-IId / blaSHV-12	rmpA / iutA / iucD / iucC / iucB / iucA	1..261706	261706

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T90385 WP_004213072.1 NZ_CP025462:214943-215386 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T90385 NZ_CP025462:214943-215386 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT90385 WP_004213073.1 NZ_CP025462:214716-214946 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT90385 NZ_CP025462:214716-214946 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620