Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1274054..1274673 Replicon chromosome
Accession NZ_CP025461
Organism Klebsiella pneumoniae strain F44

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CYE02_RS06405 Protein ID WP_002892050.1
Coordinates 1274054..1274272 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag CYE02_RS06410 Protein ID WP_002892066.1
Coordinates 1274299..1274673 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CYE02_RS06375 1270101..1270364 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
CYE02_RS06380 1270364..1270504 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
CYE02_RS06385 1270501..1271199 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
CYE02_RS06390 1271300..1272751 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
CYE02_RS06395 1272726..1273196 - 471 WP_002892026.1 YlaC family protein -
CYE02_RS31110 1273217..1273357 + 141 WP_004147370.1 hypothetical protein -
CYE02_RS06400 1273329..1273895 - 567 WP_002892030.1 maltose O-acetyltransferase -
CYE02_RS06405 1274054..1274272 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
CYE02_RS06410 1274299..1274673 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
CYE02_RS06415 1275159..1278305 - 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit -
CYE02_RS06420 1278328..1279521 - 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T90368 WP_002892050.1 NZ_CP025461:c1274272-1274054 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T90368 NZ_CP025461:c1274272-1274054 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT90368 WP_002892066.1 NZ_CP025461:c1274673-1274299 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT90368 NZ_CP025461:c1274673-1274299 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References