Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 3395294..3395927 Replicon chromosome
Accession NZ_CP025437
Organism Weizmannia coagulans strain LA204

Toxin (Protein)


Gene name mazF Uniprot ID G2TIM0
Locus tag CYJ15_RS16935 Protein ID WP_013860680.1
Coordinates 3395294..3395644 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID G2TIL9
Locus tag CYJ15_RS16940 Protein ID WP_014096808.1
Coordinates 3395649..3395927 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CYJ15_RS16895 3390550..3391329 - 780 WP_014096815.1 RNA polymerase sigma factor SigB -
CYJ15_RS16900 3391307..3391777 - 471 WP_014096814.1 anti-sigma B factor RsbW -
CYJ15_RS16905 3391781..3392116 - 336 WP_026104587.1 anti-sigma factor antagonist -
CYJ15_RS16910 3392186..3393196 - 1011 WP_017551130.1 PP2C family protein-serine/threonine phosphatase -
CYJ15_RS16915 3393210..3393611 - 402 WP_014096811.1 anti-sigma regulatory factor -
CYJ15_RS16920 3393616..3393972 - 357 WP_014096810.1 STAS domain-containing protein -
CYJ15_RS16925 3393976..3394803 - 828 WP_014096809.1 RsbT co-antagonist protein RsbRA -
CYJ15_RS16935 3395294..3395644 - 351 WP_013860680.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CYJ15_RS16940 3395649..3395927 - 279 WP_014096808.1 hypothetical protein Antitoxin
CYJ15_RS16945 3396080..3397231 - 1152 WP_110134258.1 alanine racemase -
CYJ15_RS16950 3397433..3398449 - 1017 WP_014096806.1 outer membrane lipoprotein carrier protein LolA -
CYJ15_RS16955 3398590..3398940 - 351 WP_014096805.1 holo-ACP synthase -
CYJ15_RS16960 3399221..3399820 + 600 WP_014096804.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12968.01 Da        Isoelectric Point: 4.8998

>T90332 WP_013860680.1 NZ_CP025437:c3395644-3395294 [Weizmannia coagulans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGLERDSVILLEQI
RTIDKQRLTDKITHLDEEVMEKIDDALQISLGLVEF

Download         Length: 351 bp

>T90332 NZ_CP025437:c3395644-3395294 [Weizmannia coagulans]
TTGATTGTTAAGCGTGGAGACGTGTACTTCGCCGATCTATCTCCGGTGGTCGGCTCCGAGCAGGGAGGCGTACGTCCTGT
CCTCATTTTACAAAACGACATTGGAAACCGTTTTAGCCCCACTGTCATTGTTGCCGCGATAACAGCGCAAATCCAGAAAG
CAAAATTGCCCACGCATGTGGAAATTGATGCAAAGCGTTACGGTTTGGAAAGGGATTCTGTGATATTGCTTGAACAAATA
CGCACAATCGATAAACAACGCTTAACGGATAAGATTACGCATCTTGATGAAGAAGTCATGGAAAAAATTGACGACGCCCT
GCAAATCAGCCTGGGACTGGTCGAATTTTAG

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10633.19 Da        Isoelectric Point: 5.2204

>AT90332 WP_014096808.1 NZ_CP025437:c3395927-3395649 [Weizmannia coagulans]
VSEPISTEILVRLPQQLLTELDGFAETENMNRNELIYRATKMYLKERKKRQIRETMRRGYMEMAKINLAIASEAIQAEYE
AEHTVESLVSGG

Download         Length: 279 bp

>AT90332 NZ_CP025437:c3395927-3395649 [Weizmannia coagulans]
GTGTCTGAACCTATTTCAACAGAAATTTTAGTCCGCTTGCCGCAGCAGCTGTTGACGGAATTGGACGGATTTGCTGAAAC
TGAAAATATGAATCGCAATGAATTAATTTACCGTGCCACAAAAATGTATTTAAAAGAACGGAAAAAACGCCAAATCCGCG
AAACGATGAGGCGTGGTTACATGGAAATGGCAAAAATTAATTTGGCGATTGCCTCCGAAGCGATCCAGGCTGAATACGAA
GCAGAACATACAGTTGAAAGCCTAGTAAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4BYJ0

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4BX66

References