Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 89479..90587 Replicon plasmid p2
Accession NZ_CP025427
Organism Enterococcus faecium strain SC4

Toxin (Protein)


Gene name MNTss Uniprot ID A0A0M4MHJ8
Locus tag CX663_RS16260 Protein ID WP_002303392.1
Coordinates 89479..90348 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID B9DSK1
Locus tag CX663_RS16265 Protein ID WP_002303393.1
Coordinates 90363..90587 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CX663_RS16230 84775..85026 - 252 Protein_111 GNAT family N-acetyltransferase -
CX663_RS16235 85271..86566 + 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
CX663_RS16240 86667..87575 + 909 WP_002324522.1 IS982-like element ISEfm1 family transposase -
CX663_RS16245 87578..87826 - 249 WP_002347175.1 hypothetical protein -
CX663_RS16250 87823..88731 - 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
CX663_RS16255 88764..89498 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -
CX663_RS16260 89479..90348 - 870 WP_002303392.1 nucleotidyltransferase domain-containing protein Toxin
CX663_RS16265 90363..90587 - 225 WP_002303393.1 helix-turn-helix transcriptional regulator Antitoxin
CX663_RS16270 90742..91422 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
CX663_RS16275 91631..91843 + 213 WP_002349227.1 hypothetical protein -
CX663_RS16280 92005..92523 + 519 WP_000357244.1 YfbU family protein -
CX663_RS16285 92530..93042 + 513 WP_000774078.1 hypothetical protein -
CX663_RS16290 93313..93936 + 624 WP_002288897.1 recombinase family protein -
CX663_RS16295 94032..94712 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
CX663_RS16300 94910..95515 + 606 WP_000599739.1 cell filamentation protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / aph(3')-III / ant(6)-Ia / VanHAX - 1..142988 142988
- inside IScluster/Tn erm(B) / aph(3')-III / ant(6)-Ia / VanHAX - 79678..113089 33411


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32807.38 Da        Isoelectric Point: 4.7269

>T90325 WP_002303392.1 NZ_CP025427:c90348-89479 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

>T90325 NZ_CP025427:c90348-89479 [Enterococcus faecium]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATTGTATTAGGGGGCTC
ACGTGCAAGAAGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAGTTGGATGATGAGAATAGAAACAACCTTGTTGTACCTCCCGGAGCGTGGGGTGATTGG
GTTAATGGCGGTGGATGGTTAGTTATAAACGGGTGTCATGTTGACTTGATATTACGTGATATAAAACGGGTGGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCTATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGCAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGCATTAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAATATAAACCTGAAAAATATGCCGAA
AGAGTTAATCATATTTTTGAAGTACTCGGTCTTTCTCTTTTTGAATGCTACGACATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8351.65 Da        Isoelectric Point: 4.5357

>AT90325 WP_002303393.1 NZ_CP025427:c90587-90363 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Download         Length: 225 bp

>AT90325 NZ_CP025427:c90587-90363 [Enterococcus faecium]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTTAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGATGAAATAAAAATATCTAACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MHJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M5SHM7

References