Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 81001..82138 Replicon plasmid p2
Accession NZ_CP025427
Organism Enterococcus faecium strain SC4

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag CX663_RS16205 Protein ID WP_001284311.1
Coordinates 81275..82138 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag CX663_RS16200 Protein ID WP_000301765.1
Coordinates 81001..81273 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CX663_RS16155 76210..76401 + 192 WP_002320847.1 hypothetical protein -
CX663_RS16160 76565..77152 + 588 WP_002347463.1 hypothetical protein -
CX663_RS16165 77152..78051 + 900 WP_002347464.1 RusA family crossover junction endodeoxyribonuclease -
CX663_RS16170 78051..78965 + 915 WP_002347465.1 hypothetical protein -
CX663_RS16175 79017..79232 + 216 WP_002347466.1 hypothetical protein -
CX663_RS16180 79418..79630 + 213 WP_101699790.1 hypothetical protein -
CX663_RS16185 79684..80364 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
CX663_RS16190 80420..80677 + 258 WP_002347171.1 hypothetical protein -
CX663_RS16195 80775..80984 + 210 WP_000527318.1 peptide-binding protein -
CX663_RS16200 81001..81273 + 273 WP_000301765.1 antitoxin Antitoxin
CX663_RS16205 81275..82138 + 864 WP_001284311.1 toxin zeta Toxin
CX663_RS16210 82401..83138 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
CX663_RS16215 83263..83346 - 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
CX663_RS16220 83395..83478 - 84 Protein_109 peptide-binding protein -
CX663_RS16225 83888..84682 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
CX663_RS16230 84775..85026 - 252 Protein_111 GNAT family N-acetyltransferase -
CX663_RS16235 85271..86566 + 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / aph(3')-III / ant(6)-Ia / VanHAX - 1..142988 142988
- inside IScluster/Tn erm(B) / aph(3')-III / ant(6)-Ia / VanHAX - 79678..113089 33411


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T90324 WP_001284311.1 NZ_CP025427:81275-82138 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T90324 NZ_CP025427:81275-82138 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT90324 WP_000301765.1 NZ_CP025427:81001-81273 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT90324 NZ_CP025427:81001-81273 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References