Detailed information of TA system TA090322

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 46227..46834
• Replicon: plasmid p1
• Accession: NZ_CP025426
• Organism: Enterococcus faecium strain SC4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: CX663_RS14520
• Protein ID: WP_002321032.1
• Coordinates: 46484..46834 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: CX663_RS14515
• Protein ID: WP_002287514.1
• Coordinates: 46227..46490 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CX663_RS14460	41810..41941	+	132	WP_002301801.1	single-stranded DNA-binding protein	-
CX663_RS14465	41963..42295	+	333	WP_002305808.1	single-stranded DNA-binding protein	-
CX663_RS14470	42317..42574	+	258	WP_002295625.1	hypothetical protein	-
CX663_RS14475	42733..42855	-	123	WP_002295624.1	putative holin-like toxin	-
CX663_RS14480	43045..43269	+	225	WP_074399798.1	hypothetical protein	-
CX663_RS14485	43717..43923	+	207	WP_002296239.1	hypothetical protein	-
CX663_RS14490	43923..44174	+	252	WP_002296238.1	hypothetical protein	-
CX663_RS14495	44189..44626	+	438	WP_002296237.1	hypothetical protein	-
CX663_RS14500	44619..45326	+	708	WP_002353391.1	hypothetical protein	-
CX663_RS14510	45707..45886	+	180	WP_002305761.1	hypothetical protein	-
CX663_RS14515	46227..46490	+	264	WP_002287514.1	hypothetical protein	Antitoxin
CX663_RS14520	46484..46834	+	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CX663_RS14525	46858..47472	-	615	WP_010729795.1	recombinase family protein	-
CX663_RS14530	47786..48208	+	423	WP_002288787.1	hypothetical protein	-
CX663_RS14535	48218..48421	+	204	WP_002299573.1	hypothetical protein	-
CX663_RS14540	48632..49216	+	585	WP_002299575.1	recombinase family protein	-
CX663_RS14545	49405..50085	-	681	WP_010861579.1	IS6-like element IS1216 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(2'')-Ia / aac(6')-aph(2'')	-	1..253120	253120

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T90322 WP_002321032.1 NZ_CP025426:46484-46834 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T90322 NZ_CP025426:46484-46834 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT90322 WP_002287514.1 NZ_CP025426:46227-46490 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT90322 NZ_CP025426:46227-46490 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0