Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 505174..505745 Replicon chromosome
Accession NZ_CP025425
Organism Enterococcus faecium strain SC4

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag CX663_RS02550 Protein ID WP_002286801.1
Coordinates 505404..505745 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag CX663_RS02545 Protein ID WP_002323011.1
Coordinates 505174..505404 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CX663_RS02495 500307..501638 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
CX663_RS02500 501660..502286 + 627 WP_101699687.1 cysteine hydrolase -
CX663_RS02505 502469..503050 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
CX663_RS02525 503782..504129 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
CX663_RS02530 504105..504344 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
CX663_RS02535 504549..504887 - 339 WP_002286804.1 hypothetical protein -
CX663_RS02545 505174..505404 + 231 WP_002323011.1 hypothetical protein Antitoxin
CX663_RS02550 505404..505745 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CX663_RS02555 506595..506780 + 186 WP_002304207.1 hypothetical protein -
CX663_RS02560 507285..507479 + 195 WP_002297028.1 hypothetical protein -
CX663_RS02565 507601..507919 + 319 Protein_478 transposase -
CX663_RS02575 508163..508993 - 831 WP_002286789.1 manganese catalase family protein -
CX663_RS02580 509201..510535 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T90321 WP_002286801.1 NZ_CP025425:505404-505745 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T90321 NZ_CP025425:505404-505745 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT90321 WP_002323011.1 NZ_CP025425:505174-505404 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT90321 NZ_CP025425:505174-505404 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References