Detailed information of TA system TA090319

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 1174382..1174886
• Replicon: chromosome
• Accession: NZ_CP025420
• Organism: Streptococcus parauberis strain SPOF3K

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: SPSF3K_RS05910
• Protein ID: WP_051018441.1
• Coordinates: 1174656..1174886 (+)
• Length: 77 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: SPSF3K_RS05905
• Protein ID: WP_000301765.1
• Coordinates: 1174382..1174654 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPSF3K_RS05865	1169874..1171361	+	1488	WP_010726733.1	type IA DNA topoisomerase	-
SPSF3K_RS05870	1171487..1171651	+	165	WP_002935077.1	peptide-binding protein	-
SPSF3K_RS05875	1171672..1171767	+	96	WP_001809736.1	23S rRNA methyltransferase attenuation leader peptide	-
SPSF3K_RS05880	1171892..1172629	+	738	WP_002292226.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
SPSF3K_RS05890	1172775..1173059	+	285	WP_073459356.1	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
SPSF3K_RS05895	1173162..1174058	+	897	WP_001809760.1	ParA family protein	-
SPSF3K_RS05900	1174156..1174365	+	210	WP_000527317.1	peptide-binding protein	-
SPSF3K_RS05905	1174382..1174654	+	273	WP_000301765.1	antitoxin	Antitoxin
SPSF3K_RS05910	1174656..1174886	+	231	WP_051018441.1	zeta toxin family protein	Toxin
SPSF3K_RS05915	1174921..1175601	-	681	WP_003109009.1	IS6 family transposase	-
SPSF3K_RS05925	1175674..1175829	-	156	Protein_1151	transposase	-
SPSF3K_RS05930	1176111..1177229	+	1119	WP_003104316.1	5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase	-
SPSF3K_RS05935	1177294..1178049	-	756	Protein_1153	MurR/RpiR family transcriptional regulator	-
SPSF3K_RS05940	1178163..1179095	+	933	Protein_1154	1-phosphofructokinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	erm(B) / ant(6)-Ia	-	1091411..1203560	112149
	-	inside	IScluster/Tn	erm(B) / ant(6)-Ia	-	1162706..1175829	13123

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (26-72)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 77 a.a.
• Molecular weight: 8390.46 Da
• Isoelectric Point: 4.4815

>T90319 WP_051018441.1 NZ_CP025420:1174656-1174886 [Streptococcus parauberis]
MANIVNFTDKQFENRLNDNLEELVQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKRFCCEV

Nucleotide

• Length: 231 bp

>T90319 NZ_CP025420:1174656-1174886 [Streptococcus parauberis]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAAAATCGCTTAAATGATAATTTAGAAGAATTGGTTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACGAGTTTGCGATCAGCAATTC
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACGGTTCTGTTGCGAAGTTTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT90319 WP_000301765.1 NZ_CP025420:1174382-1174654 [Streptococcus parauberis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT90319 NZ_CP025420:1174382-1174654 [Streptococcus parauberis]
ATGGCAGTTACTTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAATCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGCTATTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Structures

Antitoxin

• PDB: 3Q8X
• PDB: 1GVN
• AlphaFold DB: A0A829F0A3