Detailed information of TA system TA090312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 35070..35697
• Replicon: plasmid pBM151
• Accession: NZ_CP025409
• Organism: Paracoccus sp. BM15

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CUV01_RS18665
• Protein ID: WP_101462255.1
• Coordinates: 35290..35697 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CUV01_RS18660
• Protein ID: WP_101462254.1
• Coordinates: 35070..35300 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUV01_RS19770	30548..31216	-	669	WP_157994918.1	hypothetical protein	-
CUV01_RS18645	31216..31932	-	717	WP_101462251.1	AAA family ATPase	-
CUV01_RS18650	32532..33119	+	588	WP_198731949.1	plasmid mobilization relaxosome protein MobC	-
CUV01_RS18655	33116..34990	+	1875	WP_101462253.1	relaxase/mobilization nuclease domain-containing protein	-
CUV01_RS18660	35070..35300	+	231	WP_101462254.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CUV01_RS18665	35290..35697	+	408	WP_101462255.1	PIN domain-containing protein	Toxin
CUV01_RS18675	36281..36844	-	564	WP_101462257.1	hypothetical protein	-
CUV01_RS18680	36837..37106	-	270	WP_101462258.1	hypothetical protein	-
CUV01_RS18685	37612..38730	-	1119	WP_101462259.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92522	92522

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-116)

Antitoxin

• Antitoxin PemI-like (2-42)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15031.34 Da
• Isoelectric Point: 4.6890

>T90312 WP_101462255.1 NZ_CP025409:35290-35697 [Paracoccus sp. BM15]
MPGRFFDTNILLYLLSDDHTKADIAEGLLRGGGTVSVQVLNEIATVTQRKFRMSWAQTDEFLLMIREFVTVEPLTYETHD
LGIALARKHALSVYDAMIVAAGLLAGCDILLSEDMQDGFRVADRITIRNPFAAQI

Nucleotide

• Length: 408 bp

>T90312 NZ_CP025409:35290-35697 [Paracoccus sp. BM15]
ATGCCAGGTAGGTTCTTCGACACGAACATCCTGCTTTACTTGCTTTCTGACGACCACACCAAGGCTGACATTGCCGAAGG
GCTGCTGCGCGGCGGTGGAACCGTCAGCGTGCAGGTGCTGAACGAGATTGCGACTGTCACGCAGCGAAAATTCAGGATGA
GTTGGGCGCAGACGGATGAGTTCCTGCTCATGATCCGTGAATTTGTGACCGTCGAGCCGTTGACCTATGAGACGCATGAC
CTTGGTATCGCCCTGGCGCGGAAACACGCGCTGTCTGTCTATGACGCCATGATCGTTGCAGCCGGGCTACTGGCTGGCTG
TGACATCCTGCTGTCAGAGGACATGCAGGATGGATTCAGGGTGGCCGATAGGATCACGATCCGTAATCCATTCGCCGCGC
AAATATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8723.02 Da
• Isoelectric Point: 8.7077

>AT90312 WP_101462254.1 NZ_CP025409:35070-35300 [Paracoccus sp. BM15]
MQIAKWGNSLAVRLPARMVQELGLKAGDEIEITAADAGLFEVHRRRDPEEILARLREFRGLMPKGYRFDRDEANAR

Nucleotide

• Length: 231 bp

>AT90312 NZ_CP025409:35070-35300 [Paracoccus sp. BM15]
ATGCAGATCGCGAAATGGGGTAATTCTTTGGCCGTCCGCCTGCCCGCGCGGATGGTCCAGGAACTCGGGCTGAAGGCGGG
CGACGAGATCGAGATTACTGCGGCCGATGCCGGGCTTTTTGAGGTTCATCGACGCCGTGACCCCGAAGAAATCCTGGCTC
GCCTGCGCGAGTTTCGTGGATTGATGCCCAAGGGCTATCGGTTCGATCGGGACGAGGCGAATGCCAGGTAG