Detailed information of TA system TA090310

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 4403585..4404219
• Replicon: chromosome
• Accession: NZ_CP025407
• Organism: Streptomyces sp. CMB-StM0423

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CXR04_RS19230
• Protein ID: WP_101423604.1
• Coordinates: 4403585..4404007 (-)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2K9F4E9
• Locus tag: CXR04_RS19235
• Protein ID: WP_026276273.1
• Coordinates: 4404004..4404219 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXR04_RS19205	4399047..4400369	+	1323	WP_101423600.1	FAD-binding oxidoreductase	-
CXR04_RS19210	4400400..4401203	-	804	WP_101423601.1	hypothetical protein	-
CXR04_RS19215	4401324..4402625	-	1302	WP_101423602.1	MFS transporter	-
CXR04_RS19220	4402720..4403088	-	369	WP_101423603.1	metalloregulator ArsR/SmtB family transcription factor	-
CXR04_RS19225	4403137..4403559	-	423	WP_199850489.1	GNAT family N-acetyltransferase	-
CXR04_RS19230	4403585..4404007	-	423	WP_101423604.1	PIN domain nuclease	Toxin
CXR04_RS19235	4404004..4404219	-	216	WP_026276273.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CXR04_RS19240	4404622..4405317	+	696	WP_101423605.1	(2Fe-2S)-binding protein	-
CXR04_RS19245	4405361..4406725	-	1365	WP_101423606.1	DNA photolyase family protein	-
CXR04_RS19250	4406867..4407151	+	285	WP_199850490.1	hypothetical protein	-
CXR04_RS19255	4407212..4407628	+	417	WP_101423607.1	STAS domain-containing protein	-
CXR04_RS19260	4407811..4408707	+	897	WP_101423608.1	SigB/SigF/SigG family RNA polymerase sigma factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-49)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15243.19 Da
• Isoelectric Point: 5.6629

>T90310 WP_101423604.1 NZ_CP025407:c4404007-4403585 [Streptomyces sp. CMB-StM0423]
VNAAHYLVDTSALARMMRGDAEQYGWDRAAAAGLIAVCPITELEFLYSARSAGDRARGIEDLRTIFGWVPVDDRAYDRAL
RVQEALTKRGQHRGAGPVDLVVAATAELQGLTLLHRDRDFDCIAAATGQALQWYGPEAGK

Nucleotide

• Length: 423 bp

>T90310 NZ_CP025407:c4404007-4403585 [Streptomyces sp. CMB-StM0423]
GTGAACGCCGCGCACTACCTCGTCGACACCAGCGCACTCGCCCGGATGATGCGCGGCGACGCCGAGCAGTACGGGTGGGA
CCGCGCGGCCGCGGCCGGGCTCATCGCGGTCTGCCCCATCACCGAGCTGGAGTTCCTCTACAGCGCCCGCTCCGCCGGCG
ACCGGGCGCGGGGCATCGAAGACCTGCGGACGATCTTCGGGTGGGTGCCGGTCGACGACCGGGCCTACGACCGGGCCCTT
CGGGTGCAGGAGGCGCTGACCAAGCGCGGGCAGCACCGCGGCGCGGGTCCGGTCGACCTCGTCGTGGCGGCCACCGCGGA
GTTGCAGGGGCTGACCCTGCTGCACCGCGACCGGGACTTCGACTGCATCGCCGCCGCCACCGGGCAGGCCCTGCAGTGGT
ACGGGCCCGAGGCGGGGAAGTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7869.88 Da
• Isoelectric Point: 5.1088

>AT90310 WP_026276273.1 NZ_CP025407:c4404219-4404004 [Streptomyces sp. CMB-StM0423]
VSRTVIDLDDGLLADVAQALGTSTKKETVNTALREVLENRRRALALTRLRAAAEEGAFELDLLEDKRNYRG

Nucleotide

• Length: 216 bp

>AT90310 NZ_CP025407:c4404219-4404004 [Streptomyces sp. CMB-StM0423]
GTGAGTCGCACGGTGATCGATCTGGACGACGGCCTGCTCGCCGACGTCGCGCAGGCCCTCGGCACGAGCACCAAGAAGGA
GACGGTCAACACCGCGCTGCGCGAGGTGCTGGAGAACCGGCGGCGGGCGCTGGCGCTCACGCGGCTGCGGGCCGCGGCCG
AGGAGGGCGCGTTCGAACTCGACCTCCTTGAGGACAAGAGGAACTACCGCGGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2K9F4E9