90309

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/-
Location 95682..96058 Replicon plasmid pMS8345B
Accession NZ_CP025403
Organism Escherichia coli strain MS8345

Toxin (Protein)


Gene name parE Uniprot ID A0A075MDK3
Locus tag MS8345_RS29415 Protein ID WP_000969988.1
Coordinates 95876..96058 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID -
Locus tag MS8345_RS29410 Protein ID WP_001324224.1
Coordinates 95682..95879 (+) Length 66 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS8345_RS29935 90942..91178 + 237 WP_001323890.1 colicin V immunity protein -
MS8345_RS29395 91156..91467 + 312 WP_001259758.1 colicin V -
MS8345_RS29400 91637..93751 - 2115 WP_001183604.1 peptidase domain-containing ABC transporter -
MS8345_RS29405 93726..95000 - 1275 WP_000489608.1 HlyD family secretion protein -
MS8345_RS29410 95682..95879 + 198 WP_001324224.1 toxin-antitoxin system protein Antitoxin
MS8345_RS29415 95876..96058 + 183 WP_000969988.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MS8345_RS29420 96157..96465 + 309 WP_000377483.1 hypothetical protein -
MS8345_RS29430 97025..98059 + 1035 WP_001190234.1 3-deoxy-7-phosphoheptulonate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iucA / iucB / iucC / iucD / iutA / iroN / iroE / iroD / iroC / iroB 1..133281 133281


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6931.07 Da        Isoelectric Point: 4.5349

>T90309 WP_000969988.1 NZ_CP025403:95876-96058 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Download         Length: 183 bp

>T90309 NZ_CP025403:95876-96058 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTGTTCAGCCAGCCTCCTGTTCTCCCTTTCTTTTATCAGTGTACAGGTGC
ATGACAACATAATCACGGTATGA

Antitoxin


Download         Length: 66 a.a.        Molecular weight: 7658.53 Da        Isoelectric Point: 7.2046

>AT90309 WP_001324224.1 NZ_CP025403:95682-95879 [Escherichia coli]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Download         Length: 198 bp

>AT90309 NZ_CP025403:95682-95879 [Escherichia coli]
ATGGACCGTAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGA
CCAGGTTGAGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCAT
GGCGGGATGAAATGAGCAGGAAAGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A075MDK3

Antitoxin

Source ID Structure

References