90308

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 83552..84099 Replicon plasmid pMS8345B
Accession NZ_CP025403
Organism Escherichia coli strain MS8345

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag MS8345_RS29330 Protein ID WP_000969996.1
Coordinates 83818..84099 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag MS8345_RS29325 Protein ID WP_000079941.1
Coordinates 83552..83821 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS8345_RS29275 78826..79572 + 747 WP_000205759.1 conjugal transfer pilus acetylase TraX -
MS8345_RS29280 79627..80187 + 561 WP_000139359.1 fertility inhibition protein FinO -
MS8345_RS29285 80325..80537 + 213 WP_001311693.1 hypothetical protein -
MS8345_RS29290 80780..81181 + 402 WP_001233870.1 thermonuclease family protein -
MS8345_RS29295 81226..81486 + 261 WP_000083818.1 replication regulatory protein RepA -
MS8345_RS29305 81712..81786 + 75 WP_001365705.1 RepA leader peptide Tap -
MS8345_RS29310 81779..82636 + 858 WP_000130940.1 incFII family plasmid replication initiator RepA -
MS8345_RS29325 83552..83821 + 270 WP_000079941.1 hypothetical protein Antitoxin
MS8345_RS29330 83818..84099 + 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MS8345_RS29335 84145..84993 + 849 WP_001057989.1 3'-5' exonuclease -
MS8345_RS29340 85179..87059 + 1881 WP_001283335.1 colicin -
MS8345_RS29345 87081..87416 - 336 WP_001080729.1 colicin 1A immunity protein -
MS8345_RS29355 87545..87802 + 258 WP_000142431.1 hypothetical protein -
MS8345_RS29360 87822..88412 - 591 WP_001350923.1 hypothetical protein -
MS8345_RS29365 88409..88669 - 261 WP_000343091.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iucA / iucB / iucC / iucD / iutA / iroN / iroE / iroD / iroC / iroB 1..133281 133281


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T90308 WP_000969996.1 NZ_CP025403:83818-84099 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T90308 NZ_CP025403:83818-84099 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT90308 WP_000079941.1 NZ_CP025403:83552-83821 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT90308 NZ_CP025403:83552-83821 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References