Detailed information of TA system TA090300

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4760368..4761115
• Replicon: chromosome
• Accession: NZ_CP025401
• Organism: Escherichia coli strain MS8345

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: MS8345_RS24815
• Protein ID: WP_016239155.1
• Coordinates: 4760368..4760658 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: MS8345_RS24820
• Protein ID: WP_001285610.1
• Coordinates: 4760747..4761115 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS8345_RS24780	4755531..4757069	-	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-
MS8345_RS24790	4757904..4758464	-	561	Protein_4550	DUF4942 domain-containing protein	-
MS8345_RS24795	4758549..4758746	-	198	WP_085949158.1	DUF957 domain-containing protein	-
MS8345_RS24800	4758774..4758971	-	198	Protein_4552	hypothetical protein	-
MS8345_RS24805	4758968..4759111	-	144	Protein_4553	toxin	-
MS8345_RS24810	4759152..4760365	+	1214	WP_139371347.1	IS3 family transposase	-
MS8345_RS24815	4760368..4760658	-	291	WP_016239155.1	TA system toxin CbtA family protein	Toxin
MS8345_RS24820	4760747..4761115	-	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MS8345_RS24825	4761195..4761416	-	222	WP_000692347.1	DUF987 domain-containing protein	-
MS8345_RS24830	4761503..4761979	-	477	WP_001186193.1	RadC family protein	-
MS8345_RS24835	4761994..4762473	-	480	WP_000706978.1	antirestriction protein	-
MS8345_RS24840	4762739..4763557	-	819	WP_001175155.1	DUF945 domain-containing protein	-
MS8345_RS24845	4763647..4763880	-	234	WP_000883181.1	DUF905 family protein	-
MS8345_RS24850	4763886..4764563	-	678	WP_001097565.1	hypothetical protein	-
MS8345_RS24855	4764714..4765394	-	681	WP_001278649.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4748042..4806264	58222

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-89)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10829.41 Da
• Isoelectric Point: 5.0050

>T90300 WP_016239155.1 NZ_CP025401:c4760658-4760368 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTEPPRVSWRVFYL

Nucleotide

• Length: 291 bp

>T90300 NZ_CP025401:c4760658-4760368 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACTGAACCGCCCCGGGTTTCCTGGAGAGTGTTTTATCTGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT90300 WP_001285610.1 NZ_CP025401:c4761115-4760747 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT90300 NZ_CP025401:c4761115-4760747 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGTACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA