Detailed information of TA system TA090288

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3191108..3191762
• Replicon: chromosome
• Accession: NZ_CP025401
• Organism: Escherichia coli strain MS8345

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1PAM6
• Locus tag: MS8345_RS16640
• Protein ID: WP_000244781.1
• Coordinates: 3191108..3191515 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: MS8345_RS16645
• Protein ID: WP_016239318.1
• Coordinates: 3191496..3191762 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS8345_RS16620	3187065..3188798	-	1734	WP_000813190.1	single-stranded-DNA-specific exonuclease RecJ	-
MS8345_RS16625	3188804..3189514	-	711	WP_000715230.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
MS8345_RS16630	3189539..3190435	-	897	WP_000806987.1	site-specific tyrosine recombinase XerD	-
MS8345_RS16635	3190547..3191068	+	522	WP_001055874.1	flavodoxin FldB	-
MS8345_RS16640	3191108..3191515	-	408	WP_000244781.1	protein YgfX	Toxin
MS8345_RS16645	3191496..3191762	-	267	WP_016239318.1	FAD assembly factor SdhE	Antitoxin
MS8345_RS16650	3192005..3192985	+	981	WP_000886078.1	tRNA-modifying protein YgfZ	-
MS8345_RS16655	3193062..3193721	-	660	WP_000250274.1	hemolysin III family protein	-
MS8345_RS16660	3193885..3194196	-	312	WP_001182956.1	N(4)-acetylcytidine aminohydrolase	-
MS8345_RS16665	3194241..3195674	+	1434	WP_001350137.1	6-phospho-beta-glucosidase BglA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16061.99 Da
• Isoelectric Point: 11.5202

>T90288 WP_000244781.1 NZ_CP025401:c3191515-3191108 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T90288 NZ_CP025401:c3191515-3191108 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10573.20 Da
• Isoelectric Point: 5.1599

>AT90288 WP_016239318.1 NZ_CP025401:c3191762-3191496 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDILSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT90288 NZ_CP025401:c3191762-3191496 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACATCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: S1PAM6