Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2156179..2157010 Replicon chromosome
Accession NZ_CP025401
Organism Escherichia coli strain MS8345

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag MS8345_RS11485 Protein ID WP_000854814.1
Coordinates 2156636..2157010 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag MS8345_RS11480 Protein ID WP_001285584.1
Coordinates 2156179..2156547 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS8345_RS11445 2153083..2153538 + 456 WP_000581504.1 hypothetical protein -
MS8345_RS11450 2153617..2153850 + 234 WP_001119729.1 DUF905 family protein -
MS8345_RS11460 2153950..2154768 + 819 WP_001234629.1 DUF945 domain-containing protein -
MS8345_RS11465 2154850..2155329 + 480 WP_000860076.1 antirestriction protein -
MS8345_RS11470 2155345..2155821 + 477 WP_001186773.1 RadC family protein -
MS8345_RS11475 2155884..2156105 + 222 WP_000692323.1 DUF987 domain-containing protein -
MS8345_RS11480 2156179..2156547 + 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
MS8345_RS11485 2156636..2157010 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
MS8345_RS11490 2157007..2157201 + 195 WP_000988600.1 hypothetical protein -
MS8345_RS30050 2157214..2157327 + 114 WP_001161660.1 DUF957 domain-containing protein -
MS8345_RS11495 2157616..2157744 - 129 Protein_2125 transposase domain-containing protein -
MS8345_RS11500 2157816..2157998 + 183 WP_001016348.1 hypothetical protein -
MS8345_RS11505 2158099..2158428 - 330 WP_000450409.1 DUF496 family protein -
MS8345_RS11510 2158600..2159658 - 1059 WP_001200888.1 FUSC family protein -
MS8345_RS11515 2159856..2160329 - 474 WP_001105393.1 DNA gyrase inhibitor SbmC -
MS8345_RS11520 2160448..2161614 - 1167 WP_001513842.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T90285 WP_000854814.1 NZ_CP025401:2156636-2157010 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T90285 NZ_CP025401:2156636-2157010 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT90285 WP_001285584.1 NZ_CP025401:2156179-2156547 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT90285 NZ_CP025401:2156179-2156547 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References