Detailed information of TA system TA090276

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1611508..1613096
• Replicon: chromosome
• Accession: NZ_CP025401
• Organism: Escherichia coli strain MS8345

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B7MMY1
• Locus tag: MS8345_RS08390
• Protein ID: WP_001125475.1
• Coordinates: 1611508..1612830 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: MS8345_RS08395
• Protein ID: WP_001332145.1
• Coordinates: 1612830..1613096 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS8345_RS08350	1607294..1607527	-	234	WP_000473544.1	hypothetical protein	-
MS8345_RS08355	1607562..1607813	-	252	WP_000381239.1	membrane protein	-
MS8345_RS08360	1607816..1608133	-	318	WP_000073835.1	hypothetical protein	-
MS8345_RS08365	1608145..1609197	-	1053	WP_000568107.1	hypothetical protein	-
MS8345_RS08370	1609369..1609815	+	447	WP_000527682.1	helix-turn-helix domain-containing protein	-
MS8345_RS08375	1609915..1610337	+	423	WP_000089313.1	hypothetical protein	-
MS8345_RS08380	1610449..1610916	+	468	WP_000218697.1	hypothetical protein	-
MS8345_RS08385	1610962..1611333	+	372	WP_000795853.1	hypothetical protein	-
MS8345_RS08390	1611508..1612830	-	1323	WP_001125475.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
MS8345_RS08395	1612830..1613096	-	267	WP_001332145.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
MS8345_RS08400	1613404..1614162	+	759	WP_001286588.1	trans-aconitate 2-methyltransferase	-
MS8345_RS08405	1614166..1615080	-	915	WP_001306069.1	bestrophin family protein	-
MS8345_RS08415	1615277..1616728	-	1452	WP_001513806.1	tagaturonate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49426.24 Da
• Isoelectric Point: 8.9380

>T90276 WP_001125475.1 NZ_CP025401:c1612830-1611508 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRRNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVMCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T90276 NZ_CP025401:c1612830-1611508 [Escherichia coli]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGAGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAATGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCAAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACAGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGGCTCACACCATTTTATGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGGTCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.44 Da
• Isoelectric Point: 6.2267

>AT90276 WP_001332145.1 NZ_CP025401:c1613096-1612830 [Escherichia coli]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPNNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT90276 NZ_CP025401:c1613096-1612830 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAATGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAGTTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTAACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCAAAAAATGCCTCGCCAGAAGCA
ACAGAACAGCAAGATCTGGAGTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0E2L5M8