Detailed information of TA system TA090256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2117470..2117999
• Replicon: chromosome
• Accession: NZ_CP025395
• Organism: Staphylococcus aureus O46

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SAO46_RS10840
• Protein ID: WP_000621175.1
• Coordinates: 2117470..2117832 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SAO46_RS10845
• Protein ID: WP_000948331.1
• Coordinates: 2117829..2117999 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAO46_RS10815	2114448..2115218	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
SAO46_RS10820	2115193..2115672	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
SAO46_RS10825	2115674..2116000	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SAO46_RS10830	2116119..2117120	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SAO46_RS10840	2117470..2117832	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SAO46_RS10845	2117829..2117999	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SAO46_RS10850	2118084..2119232	-	1149	WP_001281143.1	alanine racemase	-
SAO46_RS10855	2119298..2119657	-	360	WP_000581198.1	holo-ACP synthase	-
SAO46_RS10860	2119661..2120152	-	492	WP_001205920.1	PH domain-containing protein	-
SAO46_RS10865	2120139..2121722	-	1584	WP_001294641.1	PH domain-containing protein	-
SAO46_RS10870	2121715..2122194	-	480	WP_001287083.1	hypothetical protein	-
SAO46_RS10875	2122403..2122963	-	561	WP_001092415.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T90256 WP_000621175.1 NZ_CP025395:c2117832-2117470 [Staphylococcus aureus O46]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T90256 NZ_CP025395:c2117832-2117470 [Staphylococcus aureus O46]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGATTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT90256 WP_000948331.1 NZ_CP025395:c2117999-2117829 [Staphylococcus aureus O46]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT90256 NZ_CP025395:c2117999-2117829 [Staphylococcus aureus O46]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCTATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1