Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 501876..502513 Replicon chromosome
Accession NZ_CP025341
Organism Bacillus velezensis strain CMT-6

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag CXR48_RS02430 Protein ID WP_003156187.1
Coordinates 502163..502513 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag CXR48_RS02425 Protein ID WP_003156188.1
Coordinates 501876..502157 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXR48_RS02405 498241..498840 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
CXR48_RS02410 498933..499298 + 366 WP_003156192.1 holo-ACP synthase -
CXR48_RS02415 499463..500470 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
CXR48_RS02420 500587..501756 + 1170 WP_003156189.1 alanine racemase -
CXR48_RS02425 501876..502157 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
CXR48_RS02430 502163..502513 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CXR48_RS02435 502631..503452 + 822 WP_014304404.1 STAS domain-containing protein -
CXR48_RS02440 503457..503822 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
CXR48_RS02445 503825..504226 + 402 WP_003156178.1 anti-sigma regulatory factor -
CXR48_RS02450 504238..505245 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
CXR48_RS02455 505309..505638 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
CXR48_RS02460 505635..506117 + 483 WP_003156173.1 anti-sigma B factor RsbW -
CXR48_RS02465 506083..506871 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
CXR48_RS02470 506871..507473 + 603 WP_003156170.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T90151 WP_003156187.1 NZ_CP025341:502163-502513 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T90151 NZ_CP025341:502163-502513 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT90151 WP_003156188.1 NZ_CP025341:501876-502157 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT90151 NZ_CP025341:501876-502157 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References