Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 244425..244960 Replicon plasmid unnamed
Accession NZ_CP025329
Organism Escherichia coli strain ExPEC XM

Toxin (Protein)


Gene name relE Uniprot ID V0VJJ7
Locus tag CXG97_RS28225 Protein ID WP_000222760.1
Coordinates 244673..244960 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0VIP2
Locus tag CXG97_RS28220 Protein ID WP_001132900.1
Coordinates 244425..244676 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXG97_RS28205 240361..241245 - 885 WP_000058717.1 EamA family transporter -
CXG97_RS28210 241383..241790 - 408 WP_001214976.1 cysteine hydrolase -
CXG97_RS28215 241779..243530 + 1752 Protein_250 Tn3-like element TnAs1 family transposase -
CXG97_RS28220 244425..244676 + 252 WP_001132900.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CXG97_RS28225 244673..244960 + 288 WP_000222760.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CXG97_RS28235 245400..245747 - 348 Protein_253 IS1-like element IS1A family transposase -
CXG97_RS28245 245836..246858 + 1023 WP_000255956.1 IS21-like element IS100 family transposase -
CXG97_RS28250 246855..247637 + 783 WP_001442137.1 IS21-like element IS100kyp family helper ATPase IstB -
CXG97_RS28255 247692..248056 - 365 Protein_256 IS1 family transposase -
CXG97_RS28265 248156..249377 + 1222 Protein_257 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / aph(6)-Id / aph(3'')-Ib / tet(A) vat / iroN / iroE / iroD / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA 1..286854 286854
- inside IScluster/Tn tet(A) - 234513..249115 14602


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11105.15 Da        Isoelectric Point: 10.5832

>T90142 WP_000222760.1 NZ_CP025329:244673-244960 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T90142 NZ_CP025329:244673-244960 [Escherichia coli]
ATGACTTATACAGTAAAATTCAGGGATGATGCGCTCAAAGAATGGCTGAAACTGGATAAATCCATTCAACAGCAGTTTGC
GAAAAAACTAAAGAAATGCAGCGAAAATCCTCATATTCCTTCCGCAAAACTGCGTGGATTAAAAGACTGCTACAAAATCA
AACTTCGCGCATCGGGGTTTCGTCTGGTCTATCAGGTTATTGATGACATGCTAATTATTGCGGTTGTCGCGGTAGGTAAA
CGTGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9211.41 Da        Isoelectric Point: 4.0780

>AT90142 WP_001132900.1 NZ_CP025329:244425-244676 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT90142 NZ_CP025329:244425-244676 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTATCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCGGAACTCTATGAAAGGATGC
TTGATGCGCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VJJ7

Antitoxin

Source ID Structure
AlphaFold DB V0VIP2

References