Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 147138..147685 Replicon plasmid unnamed
Accession NZ_CP025329
Organism Escherichia coli strain ExPEC XM

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag CXG97_RS27595 Protein ID WP_000969996.1
Coordinates 147404..147685 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID K4JYR0
Locus tag CXG97_RS27590 Protein ID WP_000079928.1
Coordinates 147138..147407 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXG97_RS27540 142774..142986 + 213 WP_001299730.1 ANR family transcriptional regulator -
CXG97_RS27545 143232..143693 + 462 WP_000978818.1 thermonuclease family protein -
CXG97_RS27550 143739..143948 + 210 WP_021573170.1 hemolysin expression modulator Hha -
CXG97_RS27555 143987..144577 + 591 WP_021573171.1 DUF2726 domain-containing protein -
CXG97_RS27560 144816..145076 + 261 WP_000083819.1 replication regulatory protein RepA -
CXG97_RS27570 145300..145374 + 75 WP_001375168.1 RepA leader peptide Tap -
CXG97_RS27575 145367..146224 + 858 WP_000130961.1 incFII family plasmid replication initiator RepA -
CXG97_RS27590 147138..147407 + 270 WP_000079928.1 hypothetical protein Antitoxin
CXG97_RS27595 147404..147685 + 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CXG97_RS27600 147770..148117 + 348 WP_001115718.1 hypothetical protein -
CXG97_RS27610 148331..149167 - 837 WP_000480968.1 aminoglycoside O-phosphotransferase APH(6)-Id -
CXG97_RS27615 149167..149970 - 804 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / aph(6)-Id / aph(3'')-Ib / tet(A) vat / iroN / iroE / iroD / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA 1..286854 286854


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T90132 WP_000969996.1 NZ_CP025329:147404-147685 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T90132 NZ_CP025329:147404-147685 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCACATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10175.50 Da        Isoelectric Point: 7.3210

>AT90132 WP_000079928.1 NZ_CP025329:147138-147407 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT90132 NZ_CP025329:147138-147407 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGATCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGCGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCATCTGCTGCAGCATGGAGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A5W2J4D0

References