Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 90757..91282 Replicon plasmid unnamed
Accession NZ_CP025329
Organism Escherichia coli strain ExPEC XM

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag CXG97_RS27230 Protein ID WP_001159871.1
Coordinates 90977..91282 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag CXG97_RS27225 Protein ID WP_000813630.1
Coordinates 90757..90975 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXG97_RS27190 86152..86568 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
CXG97_RS27195 86565..86795 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CXG97_RS27200 87060..87560 + 501 WP_000528932.1 hypothetical protein -
CXG97_RS27205 87573..88346 + 774 WP_000905949.1 hypothetical protein -
CXG97_RS27210 88513..89645 + 1133 Protein_76 DUF3800 domain-containing protein -
CXG97_RS27215 89679..90191 - 513 WP_000151784.1 hypothetical protein -
CXG97_RS27225 90757..90975 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CXG97_RS27230 90977..91282 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
CXG97_RS27235 91283..92089 + 807 WP_021543129.1 site-specific integrase -
CXG97_RS27240 92769..93500 - 732 WP_000504262.1 replication initiation protein -
CXG97_RS27245 94121..95326 + 1206 WP_001442122.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / aph(6)-Id / aph(3'')-Ib / tet(A) vat / iroN / iroE / iroD / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA 1..286854 286854


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T90127 WP_001159871.1 NZ_CP025329:90977-91282 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T90127 NZ_CP025329:90977-91282 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT90127 WP_000813630.1 NZ_CP025329:90757-90975 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT90127 NZ_CP025329:90757-90975 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References