Detailed information of TA system TA090123

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4776318..4777152
• Replicon: chromosome
• Accession: NZ_CP025328
• Organism: Escherichia coli strain ExPEC XM

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7MK10
• Locus tag: CXG97_RS24785
• Protein ID: WP_000854820.1
• Coordinates: 4776318..4776695 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: CXG97_RS24790
• Protein ID: WP_001285610.1
• Coordinates: 4776784..4777152 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXG97_RS24760	4772881..4774419	-	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-
CXG97_RS24770	4775254..4775814	-	561	Protein_4550	DUF4942 domain-containing protein	-
CXG97_RS24775	4775899..4776096	-	198	WP_085949158.1	DUF957 domain-containing protein	-
CXG97_RS24780	4776124..4776321	-	198	Protein_4552	hypothetical protein	-
CXG97_RS24785	4776318..4776695	-	378	WP_000854820.1	type IV toxin-antitoxin system toxin CbtA	Toxin
CXG97_RS24790	4776784..4777152	-	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CXG97_RS24795	4777232..4777453	-	222	WP_000692347.1	DUF987 domain-containing protein	-
CXG97_RS24800	4777540..4778016	-	477	WP_001186193.1	RadC family protein	-
CXG97_RS24805	4778031..4778510	-	480	WP_000706978.1	antirestriction protein	-
CXG97_RS29030	4778610..4778750	+	141	WP_000997937.1	hypothetical protein	-
CXG97_RS24810	4778776..4779594	-	819	WP_001175155.1	DUF945 domain-containing protein	-
CXG97_RS24815	4779684..4779917	-	234	WP_000883181.1	DUF905 family protein	-
CXG97_RS24820	4779923..4780600	-	678	WP_001097565.1	hypothetical protein	-
CXG97_RS24825	4780751..4781431	-	681	WP_001278649.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	papX	4765392..4838140	72748

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14043.12 Da
• Isoelectric Point: 7.8839

>T90123 WP_000854820.1 NZ_CP025328:c4776695-4776318 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Nucleotide

• Length: 378 bp

>T90123 NZ_CP025328:c4776695-4776318 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAATATTATCCTGGGTAAGCACCCGGAGGTGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT90123 WP_001285610.1 NZ_CP025328:c4777152-4776784 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT90123 NZ_CP025328:c4777152-4776784 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGTACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0E2KXX6