Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3286995..3287649 Replicon chromosome
Accession NZ_CP025328
Organism Escherichia coli strain ExPEC XM

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag CXG97_RS17195 Protein ID WP_000244781.1
Coordinates 3286995..3287402 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CXG97_RS17200 Protein ID WP_000354046.1
Coordinates 3287383..3287649 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXG97_RS17175 3282953..3284685 - 1733 Protein_3167 single-stranded-DNA-specific exonuclease RecJ -
CXG97_RS17180 3284691..3285401 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CXG97_RS17185 3285426..3286322 - 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
CXG97_RS17190 3286434..3286955 + 522 WP_001055874.1 flavodoxin FldB -
CXG97_RS17195 3286995..3287402 - 408 WP_000244781.1 protein YgfX Toxin
CXG97_RS17200 3287383..3287649 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CXG97_RS17205 3287892..3288872 + 981 WP_000886078.1 tRNA-modifying protein YgfZ -
CXG97_RS17210 3288949..3289608 - 660 WP_000250274.1 hemolysin III family protein -
CXG97_RS17215 3289772..3290083 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
CXG97_RS17220 3290128..3291561 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T90119 WP_000244781.1 NZ_CP025328:c3287402-3286995 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T90119 NZ_CP025328:c3287402-3286995 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT90119 WP_000354046.1 NZ_CP025328:c3287649-3287383 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT90119 NZ_CP025328:c3287649-3287383 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References