Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4577263..4577760 Replicon chromosome
Accession NZ_CP025318
Organism Escherichia coli strain FORC_042

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FORC42_RS24290 Protein ID WP_001173343.1
Coordinates 4577263..4577436 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag FORC42_RS24295 Protein ID WP_000398619.1
Coordinates 4577464..4577760 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC42_RS24280 4573933..4575390 + 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -
FORC42_RS24285 4575627..4577144 + 1518 WP_001295074.1 lysine--tRNA ligase -
FORC42_RS24290 4577263..4577436 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FORC42_RS24295 4577464..4577760 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FORC42_RS24305 4577895..4579108 + 1214 WP_181395482.1 IS3 family transposase -
FORC42_RS24315 4579300..4579572 - 273 WP_000405647.1 N-acetyltransferase -
FORC42_RS24320 4579584..4579814 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
FORC42_RS24325 4579995..4581626 + 1632 WP_001216487.1 sensor histidine kinase -
FORC42_RS24330 4581623..4582342 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T90095 WP_001173343.1 NZ_CP025318:c4577436-4577263 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T90095 NZ_CP025318:c4577436-4577263 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT90095 WP_000398619.1 NZ_CP025318:c4577760-4577464 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT90095 NZ_CP025318:c4577760-4577464 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References