Detailed information of TA system TA090094

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4479473..4480068
• Replicon: chromosome
• Accession: NZ_CP025318
• Organism: Escherichia coli strain FORC_042

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: -
• Locus tag: FORC42_RS23755
• Protein ID: WP_024215840.1
• Coordinates: 4479473..4479823 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: U9Y2K1
• Locus tag: FORC42_RS23760
• Protein ID: WP_001223208.1
• Coordinates: 4479817..4480068 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC42_RS23735	4474919..4475941	-	1023	WP_001296689.1	ABC transporter permease	-
FORC42_RS23740	4475955..4477457	-	1503	WP_000205813.1	sugar ABC transporter ATP-binding protein	-
FORC42_RS23745	4477597..4478553	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
FORC42_RS23750	4478863..4479393	+	531	WP_000055075.1	inorganic diphosphatase	-
FORC42_RS23755	4479473..4479823	-	351	WP_024215840.1	endoribonuclease toxin ChpB	Toxin
FORC42_RS23760	4479817..4480068	-	252	WP_001223208.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
FORC42_RS23765	4480280..4480621	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
FORC42_RS23770	4480624..4484403	-	3780	WP_000060945.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12541.47 Da
• Isoelectric Point: 5.6219

>T90094 WP_024215840.1 NZ_CP025318:c4479823-4479473 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQSAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T90094 NZ_CP025318:c4479823-4479473 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAAG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9271.58 Da
• Isoelectric Point: 4.4201

>AT90094 WP_001223208.1 NZ_CP025318:c4480068-4479817 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT90094 NZ_CP025318:c4480068-4479817 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LQ26