Detailed information of TA system TA090080

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1395802..1396427
• Replicon: chromosome
• Accession: NZ_CP025318
• Organism: Escherichia coli strain FORC_042

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FORC42_RS07395
• Protein ID: WP_000911330.1
• Coordinates: 1396029..1396427 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: FORC42_RS07390
• Protein ID: WP_000450524.1
• Coordinates: 1395802..1396029 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC42_RS07365	1391605..1392075	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
FORC42_RS07370	1392075..1392647	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
FORC42_RS07375	1392793..1393671	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FORC42_RS07380	1393688..1394722	+	1035	WP_001358397.1	outer membrane protein assembly factor BamC	-
FORC42_RS07385	1394935..1395648	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
FORC42_RS07390	1395802..1396029	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
FORC42_RS07395	1396029..1396427	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
FORC42_RS07400	1396574..1397437	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
FORC42_RS07405	1397452..1399467	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
FORC42_RS07410	1399541..1400239	+	699	WP_000679823.1	esterase	-
FORC42_RS07415	1400349..1400549	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T90080 WP_000911330.1 NZ_CP025318:1396029-1396427 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T90080 NZ_CP025318:1396029-1396427 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT90080 WP_000450524.1 NZ_CP025318:1395802-1396029 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT90080 NZ_CP025318:1395802-1396029 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33