Detailed information of TA system TA090041

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1229733..1230650
• Replicon: chromosome
• Accession: NZ_CP025308
• Organism: Bacillus velezensis strain Lzh-a42

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: CXP43_RS06455
• Protein ID: WP_007407256.1
• Coordinates: 1229904..1230650 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2C3Z8
• Locus tag: CXP43_RS06450
• Protein ID: WP_014417527.1
• Coordinates: 1229733..1229903 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXP43_RS06405	1224939..1225451	+	513	WP_012117362.1	sigma-70 family RNA polymerase sigma factor	-
CXP43_RS06410	1225564..1226223	+	660	WP_014417523.1	terminase	-
CXP43_RS06415	1226121..1226588	+	468	Protein_1183	hypothetical protein	-
CXP43_RS06420	1226601..1226972	+	372	WP_014417525.1	hypothetical protein	-
CXP43_RS06425	1226977..1227174	+	198	WP_007610833.1	XkdX family protein	-
CXP43_RS06430	1227231..1227992	+	762	WP_014417526.1	hypothetical protein	-
CXP43_RS06435	1228044..1228307	+	264	WP_003154815.1	hemolysin XhlA family protein	-
CXP43_RS06440	1228321..1228584	+	264	WP_003154813.1	phage holin	-
CXP43_RS06445	1228598..1229476	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
CXP43_RS06450	1229733..1229903	-	171	WP_014417527.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
CXP43_RS06455	1229904..1230650	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
CXP43_RS06460	1230754..1231752	-	999	WP_003154805.1	inorganic phosphate transporter	-
CXP43_RS06465	1231765..1232382	-	618	WP_003154804.1	DUF47 domain-containing protein	-
CXP43_RS06470	1232668..1233984	-	1317	WP_007610842.1	amino acid permease	-
CXP43_RS06475	1234306..1235256	+	951	WP_014417529.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T90041 WP_007407256.1 NZ_CP025308:c1230650-1229904 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T90041 NZ_CP025308:c1230650-1229904 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.78 Da
• Isoelectric Point: 10.9951

>AT90041 WP_014417527.1 NZ_CP025308:c1229903-1229733 [Bacillus velezensis]
MEQAFQNGQPEKRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT90041 NZ_CP025308:c1229903-1229733 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAAAAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2C3Z8