Detailed information of TA system TA090040

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 479750..480387
• Replicon: chromosome
• Accession: NZ_CP025308
• Organism: Bacillus velezensis strain Lzh-a42

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: CXP43_RS02445
• Protein ID: WP_003156187.1
• Coordinates: 480037..480387 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: CXP43_RS02440
• Protein ID: WP_003156188.1
• Coordinates: 479750..480031 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXP43_RS02420	476115..476714	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
CXP43_RS02425	476807..477172	+	366	WP_014304402.1	holo-ACP synthase	-
CXP43_RS02430	477337..478344	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
CXP43_RS02435	478461..479630	+	1170	WP_014416966.1	alanine racemase	-
CXP43_RS02440	479750..480031	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
CXP43_RS02445	480037..480387	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
CXP43_RS02450	480505..481326	+	822	WP_003156182.1	STAS domain-containing protein	-
CXP43_RS02455	481331..481696	+	366	WP_007609584.1	RsbT antagonist protein RsbS	-
CXP43_RS02460	481699..482100	+	402	WP_003156178.1	anti-sigma regulatory factor	-
CXP43_RS02465	482112..483119	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
CXP43_RS02470	483183..483512	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
CXP43_RS02475	483509..483991	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
CXP43_RS02480	483957..484745	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
CXP43_RS02485	484745..485347	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T90040 WP_003156187.1 NZ_CP025308:480037-480387 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T90040 NZ_CP025308:480037-480387 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT90040 WP_003156188.1 NZ_CP025308:479750-480031 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT90040 NZ_CP025308:479750-480031 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5