90035

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 535107..535644 Replicon chromosome
Accession NZ_CP025305
Organism Burkholderia pseudomallei strain R15

Toxin (Protein)


Gene name brnT Uniprot ID Q2T6G2
Locus tag R15_RS24280 Protein ID WP_004549731.1
Coordinates 535107..535376 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag R15_RS24285 Protein ID WP_004525420.1
Coordinates 535360..535644 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
R15_RS24245 530851..531891 - 1041 WP_197970191.1 ImmA/IrrE family metallo-endopeptidase -
R15_RS24250 532107..532391 - 285 WP_009961460.1 type II toxin-antitoxin system HigB family toxin -
R15_RS38155 533295..533537 - 243 WP_009961458.1 hypothetical protein -
R15_RS38160 533585..533743 - 159 WP_004549802.1 hypothetical protein -
R15_RS24260 533740..533982 - 243 WP_004545584.1 hypothetical protein -
R15_RS24270 534120..534251 - 132 WP_009923452.1 bacteriophage protein Gp48 -
R15_RS24275 534261..534536 - 276 WP_025988352.1 hypothetical protein -
R15_RS24280 535107..535376 + 270 WP_004549731.1 BrnT family toxin Toxin
R15_RS24285 535360..535644 + 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
R15_RS24300 536291..536674 + 384 Protein_440 DDE-type integrase/transposase/recombinase -
R15_RS24305 536637..537065 + 429 Protein_441 IS110 family transposase -
R15_RS24310 537292..537408 - 117 Protein_442 IS3 family transposase -
R15_RS24315 537674..539134 + 1461 WP_004547073.1 metallophosphoesterase -
R15_RS24320 539293..540555 + 1263 WP_004537187.1 c-type cytochrome -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 517132..542783 25651


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10157.46 Da        Isoelectric Point: 5.6831

>T90035 WP_004549731.1 NZ_CP025305:535107-535376 [Burkholderia pseudomallei]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Download         Length: 270 bp

>T90035 NZ_CP025305:535107-535376 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT90035 WP_004525420.1 NZ_CP025305:535360-535644 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT90035 NZ_CP025305:535360-535644 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAGGCAACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q2T6G2

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References