90032

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 536042..536579 Replicon chromosome
Accession NZ_CP025303
Organism Burkholderia pseudomallei strain PMC2000

Toxin (Protein)


Gene name brnT Uniprot ID Q2T6G2
Locus tag PMC2000_RS23870 Protein ID WP_004549731.1
Coordinates 536042..536311 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag PMC2000_RS23875 Protein ID WP_004525420.1
Coordinates 536295..536579 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PMC2000_RS23835 531786..532826 - 1041 WP_197970191.1 ImmA/IrrE family metallo-endopeptidase -
PMC2000_RS23840 533042..533326 - 285 WP_009961460.1 type II toxin-antitoxin system HigB family toxin -
PMC2000_RS38215 534230..534472 - 243 WP_009961458.1 hypothetical protein -
PMC2000_RS38220 534520..534678 - 159 WP_004549802.1 hypothetical protein -
PMC2000_RS23850 534675..534917 - 243 WP_004545584.1 hypothetical protein -
PMC2000_RS23860 535055..535186 - 132 WP_009923452.1 bacteriophage protein Gp48 -
PMC2000_RS23865 535196..535471 - 276 WP_025988352.1 hypothetical protein -
PMC2000_RS23870 536042..536311 + 270 WP_004549731.1 BrnT family toxin Toxin
PMC2000_RS23875 536295..536579 + 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
PMC2000_RS23890 537226..537609 + 384 Protein_444 DDE-type integrase/transposase/recombinase -
PMC2000_RS23895 537572..538000 + 429 Protein_445 IS110 family transposase -
PMC2000_RS23900 538227..538343 - 117 Protein_446 IS3 family transposase -
PMC2000_RS23905 538590..540050 + 1461 WP_011326543.1 metallophosphoesterase -
PMC2000_RS23910 540209..541471 + 1263 WP_004537187.1 c-type cytochrome -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 516855..537609 20754


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10157.46 Da        Isoelectric Point: 5.6831

>T90032 WP_004549731.1 NZ_CP025303:536042-536311 [Burkholderia pseudomallei]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Download         Length: 270 bp

>T90032 NZ_CP025303:536042-536311 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT90032 WP_004525420.1 NZ_CP025303:536295-536579 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT90032 NZ_CP025303:536295-536579 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAGGCAACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q2T6G2

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References