Detailed information of TA system    

experimentalExperimentally validated    

Overview


TA module


Type II Classification (family/domain) mazEF/-
Location 1230660..1231291 Replicon chromosome
Accession NC_000962
Organism Mycobacterium tuberculosis H37Rv

Toxin (Protein)


Gene name mazF Uniprot ID P9WIH9
Locus tag Rv1102c Protein ID NP_215618.1
Coordinates 1230660..1230971 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID O53451
Locus tag Rv1103c Protein ID NP_215619.1
Coordinates 1230971..1231291 (-) Length 107 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Rv1098c 1226141..1227565 - 1425 NP_215614.1 fumarate hydratase -
Rv1099c 1227596..1228684 - 1089 NP_215615.3 fructose 1,6-bisphosphatase -
Rv1100 1228683..1229384 + 702 NP_215616.1 hypothetical protein -
Rv1101c 1229391..1230548 - 1158 NP_215617.1 hypothetical protein -
Rv1102c 1230660..1230971 - 312 NP_215618.1 mRNA interferase MazF3 Toxin
Rv1103c 1230971..1231291 - 321 NP_215619.1 antitoxin MazE3 Antitoxin
Rv1106c 1232844..1233956 - 1113 NP_215622.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
Rv1107c 1233966..1234223 - 258 NP_215623.1 exodeoxyribonuclease VII small subunit -
Rv1108c 1234213..1235460 - 1248 NP_215624.1 exodeoxyribonuclease VII large subunit -
Rv1109c 1235457..1236095 - 639 NP_215625.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(8-85)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 104 a.a.        Molecular weight: 11049.76 Da        Isoelectric Point: 4.9371

>T90 NP_215618.1 NC_000962:c1230971-1230660 [Mycobacterium tuberculosis H37Rv]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNTQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T90 NC_000962:c1230971-1230660 [Mycobacterium tuberculosis H37Rv]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACACCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT90 NP_215619.1 NC_000962:c1231291-1230971 [Mycobacterium tuberculosis H37Rv]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT90 NC_000962:c1231291-1230971 [Mycobacterium tuberculosis H37Rv]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T101 Mycobacterium tuberculosis H37Rv

32.979

91.262

0.301


Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 5CCA
PDB 5UCT


Antitoxin

Source ID Structure
AlphaFold DB O53451

References


(1) Hossein Kazemian et al. (2021) Comparison of toxin-antitoxin expression among drug-susceptible and drug-resistant clinical isolates of Mycobacterium tuberculosis. Advances in Respiratory Medicine 89(2):110-114. [PubMed:33966258] experimental

(2) Jason M Schifano et al. (2013) Mycobacterial toxin MazF-mt6 inhibits translation through cleavage of 23S rRNA at the ribosomal A site. Proceedings of The National Academy of Sciences of The United States of America 110(21):8501-6. [PubMed:23650345] experimental

(3) Jeong-Sun Han et al. (2010) Characterization of a chromosomal toxin-antitoxin, Rv1102c-Rv1103c system in Mycobacterium tuberculosis. Biochemical And Biophysical Research Communications 400(3):293-8. [PubMed:20705052] experimental

(4) Amita Gupta (2009) Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiology Letters 290(1):45-53. [PubMed:19016878] experimental

(5) Ji-Li Zhao et al. (2018) Viability, biofilm formation, and MazEF expression in drug-sensitive and drug-resistant Mycobacterium tuberculosis strains circulating in Xinjiang, China. Infection And Drug Resistance 11:345-358. [PubMed:29563815] experimental


experimental experimental literature