Detailed information of TA system TA089982

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 262381..263060
• Replicon: chromosome
• Accession: NZ_CP025268
• Organism: Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: CXP41_RS01285
• Protein ID: WP_000854672.1
• Coordinates: 262381..262722 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: CXP41_RS01290
• Protein ID: WP_000070395.1
• Coordinates: 262743..263060 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXP41_RS01260	257658..258059	+	402	WP_000174689.1	sigma factor-binding protein Crl	-
CXP41_RS01265	258098..259153	-	1056	WP_000749863.1	phosphoporin PhoE	-
CXP41_RS01270	259441..260544	+	1104	WP_001285288.1	glutamate 5-kinase	-
CXP41_RS01275	260556..261809	+	1254	WP_094314171.1	glutamate-5-semialdehyde dehydrogenase	-
CXP41_RS01285	262381..262722	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
CXP41_RS01290	262743..263060	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
CXP41_RS01295	263079..263300	-	222	WP_000691994.1	DUF987 domain-containing protein	-
CXP41_RS01300	263309..263785	-	477	WP_000811693.1	RadC family protein	-
CXP41_RS01305	263801..264259	-	459	WP_000211838.1	antirestriction protein	-
CXP41_RS01310	264357..264596	-	240	WP_000194654.1	DUF905 domain-containing protein	-
CXP41_RS01315	264673..265140	-	468	WP_001547765.1	hypothetical protein	-
CXP41_RS01320	265163..265606	-	444	WP_000824223.1	hypothetical protein	-
CXP41_RS01325	265606..265833	-	228	WP_001548158.1	hypothetical protein	-
CXP41_RS01330	265829..266020	-	192	Protein_249	DeoR family transcriptional regulator	-
CXP41_RS01335	266237..267058	-	822	WP_000197389.1	DUF945 domain-containing protein	-
CXP41_RS01340	267150..268013	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T89982 WP_000854672.1 NZ_CP025268:c262722-262381 [Escherichia coli str. K-12 substr. MG1655]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T89982 NZ_CP025268:c262722-262381 [Escherichia coli str. K-12 substr. MG1655]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT89982 WP_000070395.1 NZ_CP025268:c263060-262743 [Escherichia coli str. K-12 substr. MG1655]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT89982 NZ_CP025268:c263060-262743 [Escherichia coli str. K-12 substr. MG1655]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684