89974

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 371954..372491 Replicon chromosome
Accession NZ_CP025265
Organism Burkholderia pseudomallei strain MSHR1435

Toxin (Protein)


Gene name brnT Uniprot ID A0A2K9CXB8
Locus tag CXQ84_RS23070 Protein ID WP_004557717.1
Coordinates 371954..372223 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag CXQ84_RS23075 Protein ID WP_004525420.1
Coordinates 372207..372491 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXQ84_RS38530 367949..368377 - 429 Protein_290 DDE-type integrase/transposase/recombinase -
CXQ84_RS38535 368375..369148 - 774 Protein_291 prohibitin family protein -
CXQ84_RS23035 369145..369294 - 150 WP_004542626.1 bacteriophage protein Gp44 -
CXQ84_RS23040 369550..370131 + 582 WP_004542548.1 hypothetical protein -
CXQ84_RS38540 370362..370580 - 219 WP_004552392.1 hypothetical protein -
CXQ84_RS23050 370628..370849 - 222 WP_004525418.1 hypothetical protein -
CXQ84_RS23060 370985..371116 - 132 WP_009923452.1 bacteriophage protein Gp48 -
CXQ84_RS23065 371126..371401 - 276 WP_004525419.1 hypothetical protein -
CXQ84_RS23070 371954..372223 + 270 WP_004557717.1 BrnT family toxin Toxin
CXQ84_RS23075 372207..372491 + 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
CXQ84_RS23090 373138..373521 + 384 Protein_300 DDE-type integrase/transposase/recombinase -
CXQ84_RS23095 373484..373912 + 429 Protein_301 IS110 family transposase -
CXQ84_RS23100 374139..374255 - 117 Protein_302 IS3 family transposase -
CXQ84_RS23105 374502..375962 + 1461 WP_004525423.1 metallophosphoesterase -
CXQ84_RS23110 376121..377383 + 1263 WP_004530738.1 c-type cytochrome -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 365638..379605 13967


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10183.54 Da        Isoelectric Point: 5.6831

>T89974 WP_004557717.1 NZ_CP025265:371954-372223 [Burkholderia pseudomallei]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDLMHIISMRKANKR
EVKSYVEQA

Download         Length: 270 bp

>T89974 NZ_CP025265:371954-372223 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGATGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTTGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT89974 WP_004525420.1 NZ_CP025265:372207-372491 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT89974 NZ_CP025265:372207-372491 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGTTATGACGTCGACATTGTCGACGCCTTCAAGGCAACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2K9CXB8

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References