Detailed information of TA system TA089973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 449255..449910
• Replicon: chromosome
• Accession: NZ_CP025264
• Organism: Burkholderia pseudomallei strain MSHR1435

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CXQ84_RS02560
• Protein ID: WP_004521942.1
• Coordinates: 449614..449910 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q63YL3
• Locus tag: CXQ84_RS02555
• Protein ID: WP_004202809.1
• Coordinates: 449255..449611 (-)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXQ84_RS02525	445381..446139	+	759	WP_004521940.1	cytochrome c1	-
CXQ84_RS02530	446232..446843	+	612	WP_004185176.1	glutathione S-transferase N-terminal domain-containing protein	-
CXQ84_RS02535	446913..447440	+	528	WP_004521941.1	ClpXP protease specificity-enhancing factor	-
CXQ84_RS02545	447662..448159	+	498	WP_004199888.1	terminase	-
CXQ84_RS02550	448156..449211	+	1056	WP_004557286.1	phage portal protein	-
CXQ84_RS02555	449255..449611	-	357	WP_004202809.1	putative addiction module antidote protein	Antitoxin
CXQ84_RS02560	449614..449910	-	297	WP_004521942.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CXQ84_RS02565	450578..451834	+	1257	WP_004552667.1	AAA family ATPase	-
CXQ84_RS02570	451879..452454	+	576	WP_198014201.1	DUF4276 family protein	-
CXQ84_RS02585	453226..453552	+	327	WP_004521944.1	baseplate assembly protein	-
CXQ84_RS02590	453545..453956	+	412	Protein_439	phage tail protein I	-
CXQ84_RS02595	453854..454456	+	603	WP_076857191.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	447845..455912	8067

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Uncharacterized protein HI1420 (16-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10902.68 Da
• Isoelectric Point: 9.9369

>T89973 WP_004521942.1 NZ_CP025264:c449910-449614 [Burkholderia pseudomallei]
MFKVLTTPQFDKWLDGLRDPVGSAAINLRIERAKLGNLGQWRAVGDGVNEMKIDVGPGYRAYFVRRGKIIVVVLCGGDKS
TQKKDIKLAKQIADELED

Nucleotide

• Length: 297 bp

>T89973 NZ_CP025264:c449910-449614 [Burkholderia pseudomallei]
ATGTTCAAAGTTCTGACGACCCCCCAGTTTGACAAATGGCTTGACGGGCTTCGCGATCCGGTCGGTAGCGCGGCAATCAA
CCTGCGCATCGAGCGGGCGAAGCTTGGCAATCTCGGCCAGTGGCGCGCAGTCGGCGACGGCGTCAACGAAATGAAGATTG
ATGTGGGGCCGGGATATCGGGCCTACTTCGTGCGACGCGGAAAAATTATCGTCGTGGTGTTGTGCGGCGGGGACAAGTCG
ACGCAGAAGAAGGACATCAAGCTAGCGAAGCAAATCGCTGACGAACTGGAGGATTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 12585.41 Da
• Isoelectric Point: 9.9470

>AT89973 WP_004202809.1 NZ_CP025264:c449611-449255 [Burkholderia pseudomallei]
MKISELAEFDGSKYLKDEETIRHYLAQAFEDGNPRLIQAALGNVAKARGMTALARESGVKREALYRALSEGGNAEFATIM
KVVGALGLHLTVAPAEPAPVPAPATTRARSRVRTAAHA

Nucleotide

• Length: 357 bp

>AT89973 NZ_CP025264:c449611-449255 [Burkholderia pseudomallei]
ATGAAAATCAGCGAACTGGCCGAGTTCGACGGCTCGAAGTACCTGAAGGACGAGGAAACGATTCGTCACTACCTGGCGCA
AGCGTTCGAGGATGGAAACCCGCGGCTGATTCAAGCCGCGCTCGGAAACGTCGCGAAAGCGCGCGGCATGACAGCGCTCG
CGCGCGAGTCCGGCGTGAAGCGTGAAGCGCTCTATCGCGCGCTGTCGGAAGGTGGGAACGCGGAATTCGCAACGATCATG
AAAGTTGTGGGCGCGTTGGGGCTGCACCTGACCGTTGCGCCGGCCGAACCTGCGCCGGTGCCCGCGCCGGCAACAACGCG
TGCACGCTCGCGCGTTCGCACGGCTGCGCACGCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3N4G1D3