Detailed information of TA system TA089972

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 41715..42292
• Replicon: plasmid pMF4624
• Accession: NZ_CP025260
• Organism: Listeria monocytogenes strain MF4624

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: H1G8D6
• Locus tag: CV733_RS15775
• Protein ID: WP_003728465.1
• Coordinates: 41715..42056 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H1G8D5
• Locus tag: CV733_RS15780
• Protein ID: WP_003728464.1
• Coordinates: 42050..42292 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CV733_RS15760	38194..38748	-	555	WP_003728468.1	recombinase family protein	-
CV733_RS15765	39028..39387	+	360	WP_003728467.1	Cd(II)-sensing metalloregulatory transcriptional repressor CadC	-
CV733_RS15770	39387..41522	+	2136	WP_003728466.1	cadmium-translocating P-type ATPase	-
CV733_RS15775	41715..42056	-	342	WP_003728465.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CV733_RS15780	42050..42292	-	243	WP_003728464.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CV733_RS15785	42353..42604	-	252	WP_003728463.1	hypothetical protein	-
CV733_RS15790	42735..43061	+	327	WP_003728462.1	hypothetical protein	-
CV733_RS15795	43061..43723	+	663	WP_003731676.1	DUF2786 domain-containing protein	-
CV733_RS15800	43742..44119	+	378	WP_003769263.1	DUF3850 domain-containing protein	-
CV733_RS15805	44278..44766	+	489	WP_003728520.1	hypothetical protein	-
CV733_RS15810	44888..45136	+	249	WP_003728519.1	hypothetical protein	-
CV733_RS15815	45137..45562	+	426	WP_003728518.1	hypothetical protein	-
CV733_RS15820	45566..46288	+	723	WP_003728517.1	CRISPR-associated protein Cas5	-
CV733_RS15825	46304..47020	+	717	WP_003731678.1	AP2 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..63182	63182

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-128)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 16796.15 Da
• Isoelectric Point: 5.5500

>T89972 WP_003728465.1 NZ_CP025260:c42056-41715 [Listeria monocytogenes]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF

Nucleotide

• Length: 342 bp

>T89972 NZ_CP025260:c42056-41715 [Listeria monocytogenes]
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTT

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 11939.32 Da
• Isoelectric Point: 5.5500

>AT89972 WP_003728464.1 NZ_CP025260:c42292-42050 [Listeria monocytogenes]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
F

Nucleotide

• Length: 243 bp

>AT89972 NZ_CP025260:c42292-42050 [Listeria monocytogenes]
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTT

Structures

Toxin

• AlphaFold DB: H1G8D6

Antitoxin

• AlphaFold DB: H1G8D5