Detailed information of TA system TA089971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3435864..3436781
• Replicon: chromosome
• Accession: NZ_CP025258
• Organism: Bacillus sp. SJ-10

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: CXL14_RS16810
• Protein ID: WP_007407256.1
• Coordinates: 3435864..3436610 (+)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2C3Z8
• Locus tag: CXL14_RS16815
• Protein ID: WP_014417527.1
• Coordinates: 3436611..3436781 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXL14_RS16790	3431258..3432208	-	951	WP_014417529.1	ring-cleaving dioxygenase	-
CXL14_RS16795	3432530..3433846	+	1317	WP_007610842.1	amino acid permease	-
CXL14_RS16800	3434132..3434749	+	618	WP_003154804.1	DUF47 domain-containing protein	-
CXL14_RS16805	3434762..3435760	+	999	WP_003154805.1	inorganic phosphate transporter	-
CXL14_RS16810	3435864..3436610	+	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
CXL14_RS16815	3436611..3436781	+	171	WP_014417527.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
CXL14_RS16820	3437038..3437916	-	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
CXL14_RS16825	3437930..3438193	-	264	WP_003154813.1	phage holin	-
CXL14_RS16830	3438207..3438470	-	264	WP_003154815.1	hemolysin XhlA family protein	-
CXL14_RS16835	3438522..3439283	-	762	WP_014417526.1	hypothetical protein	-
CXL14_RS16840	3439340..3439537	-	198	WP_007610833.1	XkdX family protein	-
CXL14_RS16845	3439542..3439913	-	372	WP_014417525.1	hypothetical protein	-
CXL14_RS16850	3439926..3440393	-	468	Protein_3221	hypothetical protein	-
CXL14_RS16855	3440291..3440950	-	660	WP_014417523.1	terminase	-
CXL14_RS16860	3441063..3441575	-	513	WP_012117362.1	sigma-70 family RNA polymerase sigma factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T89971 WP_007407256.1 NZ_CP025258:3435864-3436610 [Bacillus sp. SJ-10]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T89971 NZ_CP025258:3435864-3436610 [Bacillus sp. SJ-10]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.78 Da
• Isoelectric Point: 10.9951

>AT89971 WP_014417527.1 NZ_CP025258:3436611-3436781 [Bacillus sp. SJ-10]
MEQAFQNGQPEKRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT89971 NZ_CP025258:3436611-3436781 [Bacillus sp. SJ-10]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAAAAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2C3Z8