Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 178011..178648 Replicon chromosome
Accession NZ_CP025258
Organism Bacillus sp. SJ-10

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag CXL14_RS00975 Protein ID WP_003156187.1
Coordinates 178011..178361 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag CXL14_RS00980 Protein ID WP_003156188.1
Coordinates 178367..178648 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXL14_RS00935 173051..173653 - 603 WP_007410234.1 SpoIIE family protein phosphatase -
CXL14_RS00940 173653..174441 - 789 WP_003156171.1 RNA polymerase sigma factor SigB -
CXL14_RS00945 174407..174889 - 483 WP_007609591.1 anti-sigma B factor RsbW -
CXL14_RS00950 174886..175215 - 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
CXL14_RS00955 175279..176286 - 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
CXL14_RS00960 176298..176699 - 402 WP_003156178.1 anti-sigma regulatory factor -
CXL14_RS00965 176702..177067 - 366 WP_007609584.1 RsbT antagonist protein RsbS -
CXL14_RS00970 177072..177893 - 822 WP_003156182.1 STAS domain-containing protein -
CXL14_RS00975 178011..178361 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CXL14_RS00980 178367..178648 - 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
CXL14_RS00985 178768..179937 - 1170 WP_053285486.1 alanine racemase -
CXL14_RS00990 180054..181061 - 1008 WP_021495080.1 outer membrane lipoprotein carrier protein LolA -
CXL14_RS00995 181226..181591 - 366 WP_014304402.1 holo-ACP synthase -
CXL14_RS01000 181684..182283 + 600 WP_003156193.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T89970 WP_003156187.1 NZ_CP025258:c178361-178011 [Bacillus sp. SJ-10]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T89970 NZ_CP025258:c178361-178011 [Bacillus sp. SJ-10]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT89970 WP_003156188.1 NZ_CP025258:c178648-178367 [Bacillus sp. SJ-10]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT89970 NZ_CP025258:c178648-178367 [Bacillus sp. SJ-10]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References